Research Technician; Polizzi Lab

Overview

The Polizzi lab uses a mix of computational and experimental approaches to design and characterize ligand-binding proteins. We develop machine-learning techniques to compute the structures and sequences of proteins that bind to challenging small-molecule ligands. We then express these proteins in the lab and characterize them with biophysical and structural methods. We aim not only to unlock the design principles used by proteins for molecular recognition but also to make useful proteins for probing and manipulating biology.

By designing de novo small-molecule-binding proteins, we can isolate the essential ingredients for protein molecular recognition events, obtain atomic-level control of the protein/small-molecule interface, and set the stage for the development of proteins that could serve as novel sensors, enzymes, drug delivery vehicles, or pharmaceuticals.

The lab is located at Dana-Farber Cancer Institute and Harvard Medical School, with access to state-of-the-art facilities including cryo-EM, X-ray crystallography, flow cytometry, and high-performance computing. We collaborate with leading biologists and physician scientists to pursue high-impact applications of protein design for understanding and controlling biology.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in cancer research and patient care. We strive to create an inclusive, diverse, and equitable environment and to promote public health, particularly among high-risk and underserved populations.

A research technician position is available in the lab to contribute to experimental pipelines for the characterization of designed small-molecule binding proteins. This entry-level role offers the opportunity to gain experience in a cutting-edge protein design laboratory. We seek enthusiastic individuals with strong interpersonal skills, a growth mindset, excellent organization, and the ability to work collaboratively in a team.

• Protein expression and purification
• Cloning and mutagenesis
• Flow cytometry and yeast protein surface display
• Assist in structure determination by cryo-EM and/or X-ray crystallography
• Perform binding studies using SPR, BLI, fluorescence polarization, calorimetry, etc.
• Attend team meetings to share results
• Document findings in a well-managed lab notebook and in oral/written reports
• Set up, adjust, calibrate, clean, maintain and troubleshoot equipment
• Clean, maintain and prepare supplies and work areas; may be responsible for ordering of supplies
• Collaborates with Purchasing and may be responsible for negotiating with vendors
• Conduct standardized biological, microbiological or biochemical tests and laboratory analyses
• Isolate, identify and prepare specimens for examination
• Monitor laboratory work to ensure compliance with Environmental Health and Safety information and SOPs
• Provide intellectual and interactive commitment to position by keeping up with appropriate literature and publications
• May be responsible for development, modification and improvement of standard operating procedures
• Use computers, computer-interfaced equipment, robotics or high-technology applications to perform duties
• May be responsible for designing and executing advanced experiments and setting strategy

• Knowledge of information and techniques related to biology, chemistry, or medicine is preferred
• Excellent communication skills (written and verbal) and ability to work with a wide variety of faculty and staff
• Ability to analyze information and use logic to address work-related issues
• Attention to detail and thoroughness in completing tasks
• Ability to work within a team environment

The position requires a bachelor’s degree, preferably in biology, biophysics, biochemistry, computer science, or related fields, with no prior professional experience necessary. We are looking for candidates available for at least 2 years.

No supervisory responsibilities. Normally receives detailed instructions on all work.

None

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong. We are committed to having faculty and staff who offer multifaceted experiences. Cancer knows no boundaries, and we are dedicated to hiring the most qualified and compassionate professionals.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

Pay Transparency Statement:
The hiring range is based on market pay structures, with salaries determined by factors such as business…