Research Assistant II- Ebrahimi-Fakhari Laboratory

Position: Research Assistant II-The Ebrahimi-Fakhari Laboratory
Job Posting Description

Position Summary/ Department Summary:
The Ebrahimi-Fakhari Laboratory within the F.M. Kirby Neurobiology Center at Boston Children's Hospital is seeking a highly motivated Research Assistant II to support translational neuroscience research focused on rare childhood-onset neurogenetic disorders, including hereditary spastic paraplegia.
The laboratory integrates patient-derived cellular systems, stem cell-based neuronal models, brain organoids, mouse models, and gene-based therapeutic development to advance disease-modifying strategies. The Research Assistant II will contribute to both experimental research (approximately 50%) and laboratory operations and regulatory coordination (approximately 50%).
This position is ideal for candidates seeking rigorous research training in a collaborative academic medical center and who are pursuing long-term careers in biomedical research, biotechnology, laboratory leadership, or graduate/medical training. A two-year commitment is strongly preferred.

Key Responsibilities:

• Perform and optimize molecular and cellular biology techniques (PCR, Western blotting, immunostaining, protein analysis)
• Maintain and support patient-derived fibroblast and neuronal cell culture systems
• Assist in design and execution of cellular trafficking and neurobiology assays
• Support mouse colony management, genotyping, and experimental procedures
• Analyze, organize, and present experimental data
• Coordinate day-to-day laboratory operations and workflow efficiency
• Maintain laboratory inventory systems, reagent databases, and procurement
• Monitor cryostorage systems and ensure accurate sample tracking
• Support preparation and renewal of animal and biosafety protocols
• Assist with regulatory compliance, safety procedures, and controlled substance documentation
• Serve as liaison with collaborators, vendors, and institutional offices
• Contribute to a collaborative and inclusive research environment

Minimum Qualifications
Education:

• Bachelor's degree in neuroscience, biology, biochemistry, molecular biology, or a related scientific discipline required.

Experience:

• Minimum of 1-2 years of relevant laboratory research experience is required.
• Demonstrated proficiency in molecular and cellular biology techniques required.
• Experience with mammalian cell culture, patient-derived cell systems, or mouse models preferred.
• Experience working in a regulated research environment and supporting laboratory operations preferred.