Contractor, Protein Sciences

Job Title: Contractor, Protein Sciences

Job Number: 37367

Location: Boston,MA

We are looking for a driven scientist to join the team at our Boston, MA laboratories as a contractor. In this role, the individual will be part of the Structural Biology and Protein Sciences group, contributing to the production of high-quality recombinant proteins to support assay development, screening, and structural biology projects. The ideal candidate will have prior experience expressing proteins in bacterial, insect, and/or mammalian systems, along with familiarity in standard protein purification techniques using both batch methods and liquid chromatography.

They should also be comfortable learning and carrying out new protocols with guidance and training provided in-house.

• Prepare and sterilize laboratory media and buffer solutions
• Culture and maintain insect and mammalian cells under sterile conditions, and generate baculovirus stocks
• Carry out transformations and transfections to produce recombinant proteins in bacterial, insect, and/or mammalian systems
• Purify expressed proteins using a range of chromatography techniques and evaluate their quality with standard assays (e.g., SDS-PAGE, Western blot, DLS, nano
  
  DSF, aSEC, intact mass spectrometry)
• Support routine instrument setup, maintenance, and cleaning
• Contribute to additional protein science activities such as forming protein–ligand and protein–protein complexes, as well as protein crystallization
• Interpret, summarize, and record experimental data using the internal electronic lab notebook system
• Communicate effectively to manage priorities and expectations
• Oversee lab inventory and coordinate ordering of supplies

• Bachelor’s or Master’s degree in biochemistry, cell biology, or a closely related discipline
• Solid understanding of standard cell and molecular biology lab techniques and equipment
• Hands‑on experience preparing media and buffer solutions
• Experience isolating and purifying recombinant proteins from bacterial, insect, and/or mammalian expression systems
• Foundational knowledge of chromatography methods (e.g., affinity, ion exchange, size exclusion) and related systems such as batch setups and FPLC instruments (e.g., ÄKTA)
• Familiarity with common protein analysis techniques mentioned above
• Highly self‑driven with a strong interest in protein biochemistry and function
• Able to perform work accurately in sterile environments
• Strong communication skills
• Well‑organized with keen attention to detail

Salary: $35-40/hour