Research Lab Tech

Technical Research Assistant I

The Technical Research Assistant I will conduct research on epithelial barrier regulation, tight junction biology, and mucosal immune mechanisms in the gastrointestinal tract. Reporting to the Principal Investigator (PI), the Research Assistant will perform experiments designed in collaboration with the PI and other lab members. The degree of independence will be determined based on knowledge, technical ability, and understanding of the specific project.

While performing research, the Research Assistant will also support aspects of laboratory operations. The research project may be substantially in vitro- or in vivo-based or combine in vitro and in vivo approaches. This position requires a Bachelor's degree in biological or related sciences. Post-baccalaureate research experience is preferred. The successful candidate will demonstrate growing scientific independence, strong technical skills, problem-solving ability, and commitment to rigorous experimental documentation.

• Independent Research Project
• Drive a defined research project investigating epithelial barrier function, tight junction regulation, or mucosal immune responses using cell culture models and/or mouse models.
• Design and perform experiments in collaboration with the PI.
• Troubleshoot experimental challenges with input from PI and other laboratory personnel.
• Analyze and interpret data; prepare figures and contribute to presentations and manuscripts.
• Present findings at laboratory meetings and participate actively in scientific discussions.
• Technical Support and Laboratory Operations
• Perform experiments and provide technical support for ongoing laboratory projects, including work with other lab members.
• Maintain mammalian cell lines (e.g., Caco-2, MDCK, T84) and/or primary intestinal epithelial stem cells, including transwell culture systems and barrier function assays, routine passage, quality control, and cell banking.
• Execute established techniques including transwell barrier assays, immunofluorescence, Western blotting, and qPCR.
• Execute mouse-based techniques as needed, including tissue collection, processing, histologic and immunofluorescence analysis, and functional assays.
• Assist with mouse colony management, including routine husbandry, genotyping, breeding support, and record-keeping.
• Maintain laboratory reagents, equipment, and shared resources in good working order.
• Help train new laboratory members.
• Documentation and Resource Management
• Maintain detailed, high-quality documentation of all experimental work in laboratory notebooks and electronic systems.
• Contribute to maintenance of databases of mice, cell lines, reagents, and other materials.
• Learn and help implement new experimental methods and protocols.
• Scientific Development and Collaboration
• Participate actively in laboratory meetings and journal clubs, developing critical thinking and scientific communication skills.
• Read relevant scientific literature to inform your project and stay current with the field.
• Work collaboratively with other lab members.
• Assist with preparation of figures, data summaries, and methods sections for manuscripts.

Education

• Bachelor's degree in Biology, Immunology, Molecular Biology, Cell Biology, Biomedical Sciences, or a closely related field is required.

Experience

• Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred.

Knowledge,

Skills and Abilities

• Strong theoretical and practical knowledge of laboratory techniques and procedures.
• Familiarity with common laboratory equipment and instruments used in research experiments.
• Proficiency in data collection, analysis, and scientific software tools.
• Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively.
• Strong problem-solving skills and the ability to troubleshoot technical issues.
• Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges.

Additional Job Details (if applicable)

• Remote Type:
  Onsite
• Work Location:
  
  20 Shattuck Street
• Scheduled Weekly
  
  Hours:
  
  40
• Employee Type:
  Regular
• Work Shift:
  
  Day (United States of America)
• Pay Range: $21 - $29.01/Hourly
• Grade: 5

At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications;

however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth.…