Research Assistant Neurology KL

Job Summary

The candidate will work independently under the supervision of Vikram Khurana, MD, PhD, and a senior researcher in the Khurana lab. Depending on the specific project needs and candidate skill set, the role will range from molecular biology focused bench work to development and optimization of assays for viability/toxicity in iPSC derived neurons. The candidate is expected to be diligent, highly organized, and collegial.

• Maintenance, passaging, cataloguing, and culturing of iPSC cells.
• Differentiation of iPSCs to neurons and glia and aging of these cultures.
• Maintenance of Attune flow cytometry and ordering related reagents.
• Measuring and scheduling longitudinal imaging with Incucyte and Nikon Biostation microscope.
• Maintenance of HEK
  293 cells and generation of stable cell lines.
• Assisting with molecular cloning;
  Gibson assembly, gateway cloning, mini/maxi preps.
• Analysis of data from western blot/microscopy/flow cytometry.
• Maintaining lab note‑keeping habits (mostly digital, Lab Archives) and generating standard operating procedures.
• Organizing experiments, labeling, and file management.
• Reporting daily to the immediate supervisor and weekly to the Principal Investigator.
• Interacting with junior and senior members of the lab to troubleshoot experiments.
• Sharing lab duties with other technicians.
• Responsible for data collection, manipulation, analysis, and reporting.
• Managing the study cohort.
• Outreach communication with subjects, conducting mailings, follow‑up, and obtaining necessary documents.
• Assisting with epidemiological studies and other population‑based research.
• Performing additional duties as assigned.
• Complying with all policies and standards.

Bachelor's Degree in Science required. This role may accept experience in lieu of a degree.

• New graduate with 0‑1 year of relevant coursework (preferred).
• Good cell culture experience with human cells (HEK
  293 or any other immortalized line).
• iPSC stem cell culture experience is a plus but not required.
• Excellent cell culture practice and sterile techniques are necessary.
• Medium to high level of computer literacy and basic understanding of statistical concepts.
• Basic understanding of molecular techniques, microscopy, flow cytometry.
• Understanding of basic molecular concepts: cloning, western blot, RNA isolation, qPCR.

• Excellent oral and written communication skills.
• Strong interpersonal skills for working in a diverse lab.
• Excellent organizational skills and ability to prioritize tasks.
• Excellent data management skills and attention to detail.
• Good presentation skills are a plus.
• Computer literacy, including database tools.
• Ability to follow directions and exhibit professionalism.

Remote Type:
Onsite.

Work Location:

60 Fenwood Road. Scheduled Weekly

Hours:

40. Employee Type:
Regular Work Shift Day (United States of America). Pay Range: $29.01 per hour. Grade: 5.

The Brigham and Women's Hospital, Inc. is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will provide reasonable accommodation to individuals with disabilities. Applicants who require accommodation may contact Human Resources at .