Principal Scientist, Protein Engineering

Alta Resource Technologies, Inc. Principal Scientist, Protein Engineering

Location:

Boulder, CO

Reports To:

Sr. Director of Applied Biology About Us Alta is reinventing how the world sources critical minerals — using protein-based chemistry to extract rare earth elements and other essential materials from challenging feed stocks with unmatched selectivity. By dramatically reducing the cost and environmental impact of mineral separation, Alta is making it possible to unlock new sources of supply for 21st-century technologies. As global demand accelerates, securing these materials has become one of the most urgent challenges facing U.S. competitiveness and national security.

Backed by top investors and government partners, Alta is growing fast and hiring mission-driven scientists and engineers to help us scale our platform and transform the future of mining. Role Summary The Principal Scientist in Protein Engineering will oversee the scientific process optimizations for the directed evolution of metalloprotein variants, including implementing state-of-the-art approaches to protein design, expression, and testing of proteins for continuous improvement.

The role will execute at the bench to develop workflows for protein design, cloning, expression, and assays to enhance protein production, purification, and downstream applications. The candidate will have experience in agile process workflows to implement directed evolution campaigns that will yield proteins with novel characteristics. The candidate will develop robust protocols for transfer within internal and external groups. The individual will join a group of collaborative scientists with diverse skill sets and must have strong communication and critical thinking skills with a rigorous approach to science.

Key Responsibilities 1. Develop and optimize high throughput protein engineering workflows
• Establish construct design and cloning workflows that enable high-throughput and multiplexed approaches for protein variant testing and selections.
• Develop high-throughput variant libraries for improving strain and protein performance including optimizing for protein production, aKnity, specificity, and stability.
• Develop and implement automation and experimental strategies to support high- throughput and library-scale directed evolution campaigns.
• Collaborate with the team to develop end-to-end processes designed for workflow eKciency.
2. Develop and implement scale-up protein production workflows
• Apply data analysis from directed evolution campaigns to inform scale-up production of lead protein candidates.
• Design and implement protein production improvements to achieve round-over- round improvement.
3. Leadership and Communication
• Ensure excellent practices in documentation of all protocols and experiments.
• EMectively communicate results with stakeholders across the company.
• Develop talent through team building and project execution.

Required Qualifications
● Ph.D. in biochemistry, molecular biology, or related field with 10+ years of industry experience.
● Expertise in high-throughput molecular biology approaches and library-scale workflows.
● Expertise in protein engineering, including novel protein design, scalable microtiter production and assays, informed data interpretation for protein candidate selections.
● Expertise in design and implementation of automated workflows.
● Experience in microbial bioproduction.
● Lead and contribute at the bench with hands-on workflow development.
● Deep knowledge of classic and state-of-the-art molecular biology and multiplex library- scale approaches to directed evolution campaigns.
● Demonstrated knowledge of experimental design, the scientific method, and statistical analysis.
● Strong written and verbal communication skills and ability to work within di Merent teams.
● Strong organizational and documentation skills.

Preferred Qualifications
• Experience with metalloprotein biochemistry.
• Experience with bioconjugate chemistry.
• Experience with fermentation and down-stream processing.
• Proficiency in working with data science teams for scripting pipelines and bioinformatics. Compensation…