Research Scientist I​/II, Oligonucleotide Chemistry

Role Summary

This on-site role is located in Cambridge, MA, with no remote work or relocation assistance. Local candidates only will be considered. The Research Scientist I/II will join the xRNA Therapeutics team to perform hands‑on oligonucleotide synthesis, purification, and analysis, supporting the growth of internal synthesis capabilities under the supervision of a Principal Scientist. The role requires a proactive, collaborative approach in a fast‑paced, interdisciplinary environment.

• Execute solid‑phase synthesis of research‑scale oligonucleotides with various chemical modifications using automated DNA/RNA synthesizers such as Shasta, K&A, and Mermade.
• Perform purification, desalting, and analysis of oligonucleotides using techniques including reverse‑phase, ion‑exchange HPLCs, UPLCs, and LCMS.
• Method development and optimization for improved synthesis, purification and analysis of oligonucleotides and conjugates.
• Prepare and maintain standard operating procedures (SOPs) for synthesis, processing, and purification of therapeutic oligonucleotides to ensure reproducibility.
• Operate, troubleshoot, and maintain critical laboratory equipment such as synthesizers, HPLCs, and LCMS.
• Organize experimental data and maintain a well‑documented electronic notebook (eLN).
• Demonstrate high motivation and productivity in a fast‑paced environment.

• Preferred:
  Research Scientist I level – at least 1 year of chemistry laboratory experience in academic or industrial settings.
• Preferred:
  Research Scientist II level – at least 2 years of chemistry laboratory experience in academic or industrial settings.
• Required:
  
  Familiarity with automated solid‑phase synthesizers and therapeutic oligonucleotide synthesis and downstream processing.
• Required:
  
  Proficiency in purifying, analyzing and characterizing oligonucleotides via LC/HRMS, RP‑HPLC, AEX‑HPLC, SEC‑HPLC, and UV‑vis analytics.
• Required:
  
  Basic understanding of RNA therapeutics and their role in biology.
• Required:
  
  Be a team player prepared to work in a collaborative, fast‑paced environment with a high degree of flexibility and self‑motivation.

• Interdisciplinary collaboration and ability to work in a fast‑paced environment.

• Bachelor’s degree in chemistry or related field.