Research Investigator, Mass Spectrometry

Position Summary:

We are seeking a highly skilled and motivated full time Sr Research Investigator/ Research Investigator. The successful candidate will play a key role in supporting discovery stage programs with a particular focus on targeted protein degradation and next generation biologic therapeutics.

The ideal candidate will possess extensive hands-on experience in mass spec-based bioanalysis, protein and peptide characterization, and analytical assay development.

This is a cross-functional role with significant impact on the research and development pipeline.

Key Responsibilities:

* Lead the development, optimization, and fit for purpose validation of LC-MS/MS assays and IP (hybrid ligand binding) assay for quantification of biomarkers, proteins, peptides, cytokines, and metabolites

* Design and execute workflows for peptide mapping, post-translational modification (PTM) analysis, disulfide bond mapping, and glycosylation analysis

* Develop fit-for-purpose bioanalytical methods for proteins with complex attributes (e.g., high MW, high glycosylation, unexpected MWs) using advanced enzymatic digestion strategies

* Determine and communicate goals, timelines, and priorities for bioanalysis in cross-functional project teams

* Conduct advanced data processing and statistical analysis using multiple software from different vendors

* Maintain and troubleshoot analytical instrumentation

* Maintain technical reports, SOP, and an Electronic notebook

Required Qualifications:

* Minimum of 5 years of relevant industry experience

* Extensive experience with mass spectrometry analysis of proteins and peptides (Sciex QTRAP, Sciex Q-TOF, Thermo Orbitrap Fusion/Lumos, QQQ with MRM method)

* Demonstrated expertise in bioanalytical method development and validation of large molecule analytes

* Experience performing intact and subunit protein mass spectrometry analysis, including characterization of monoclonal antibodies, fusion proteins

* Hands-on experience with a range of chromatography techniques RP-HPLC, SEC, HILIC, nanoLC, microLC)

* Proficient in wet lab sample preparation techniques (SPE, IP, LLE)

* Ability to maintain and troubleshoot mass spec instrumentation in close collaboration with vendors to ensure optimal system performance and uptime

* Excellent organizational skills and attention to detail

* Ability to balance multitasks with competing priorities

* Strong written and verbal communication skills

Work Environment:

* Laboratory-based position (based in Cambridge)

* Fast paced enviroment

* Collaboration with cross-functional R&D and bioanalytical teams

Preferred Qualifications:

* Experience with computational biology, bioinformatics

* Experience with biochemical, biophysical, biology assays such as SPR, TR-FRET, ELISA, MSD

* Experience executing method development, validation, and assay transfer at CROs and external partners