Research Scientist I - Protein Sciences and Structural Biology

Embedded within the Broad Institute, the Center for the Development of Therapeutics (CDoT) is a highly collaborative organization of professional drug discovery scientists. Our mission is to translate cutting‑edge biological insights originating from the Broad community into impactful drug discovery projects. We partner with faculty from MIT, Harvard, and the Harvard teaching hospitals together with industry partners in pharmaceutical, biotechnology and venture capital firms to validate druggable targets and identify leads.

We combine our deep expertise in drug discovery with the knowledge and unique capabilities of the Broad Institute to explore drug discovery projects in a wide range of disease areas (including oncology, cardiovascular, psychiatry, infectious and rare diseases). We use systematic approaches in genomics, biology, high‑content imaging, chemistry, and machine learning coupled with the Broad's culture of creativity, risk taking, and open sharing, to successfully advance science and develop new treatments for patients.

• Develop and execute strategies to clone and express recombinant proteins in a variety of expression systems.
• Purify tagged or native proteins using standard purification (FPLC systems and column chromatography) and analytical techniques (SDS‑PAGE, Western blot, light scattering, and protein LC/MS).
• With training, produce high‑quality protein crystal structures and co‑crystal structures, carrying out the process from crystallization screening through to structure refinement.
• Initiate and complete routine protein construct design and production in an independent and efficient manner.
• Interpret experimental outcomes and propose follow‑up studies.
• Troubleshoot and solve scientific problems related to protein production independently.
• Operate and maintain laboratory equipment independently, including protein purification systems such as an AKTA Pure.
• Investigate new technologies related to protein expression and purification.
• Document work and effectively present results in cross‑functional project teams.

• Ph.D. in Biophysics, Biochemistry, Molecular Biology, Structural Biology, or a related life science field with 0–2+ years of research experience in an industrial or academic setting.
• Expertise in recombinant protein purification using chromatography (affinity, ion exchange, and size exclusion).
• Significant experience with cloning and expressing proteins using different vectors, tags, and various cell culture systems (mammalian, insect, bacterial, yeast).
• Significant experience with protein characterization (analytical size exclusion chromatography, mass spectrometry, light scattering, western blot, and biophysical binding studies).
• Experience in protein crystallography and crystallographic data processing, model refinement, and structural analysis is a plus.
• Experience with biophysical characterization of proteins (ITC, DSC, SPR, NMR, etc.) is a plus.
• A proven record of successful interaction with project team members, external customers, or vendors.

The successful candidate will be a results‑focused, team‑oriented, creative bench scientist able to excel in a fast‑track environment. She or he must be self‑motivated, detail‑oriented, and exhibit strong problem‑solving skills. In addition, the successful candidate should be a clear and effective communicator, demonstrating strong interpersonal skills to enable collaboration with project teams, peers, and management in identifying and achieving research goals.

From day one, this role offers a competitive benefits package including medical, dental, vision, life, and disability insurance; a 401(k) retirement plan; flexible spending and health savings accounts; at least 13 paid holidays; winter closure; paid time off; parental and family care leave; and an employee assistance program, among other Broad benefits.

The Broad Institute is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, national origin, religion, age, color, sex, disability, protected veteran status, or any other characteristic protected by local, state, or federal laws, rules, or regulations.

Should you need a reasonable accommodation to complete the application or interview process, please contact recruiting for assistance.