Research Technician II, Gene Editing Core

Research Technician II

The Gene and Cell Therapy Institute (GCTI) at Mass General Brigham is seeking a highly motivated Research Technician II to support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and associated molecular and cellular workflows. The successful candidate will perform mammalian cell culture, molecular biology, genome editing, and quality-control assays in support of research projects from investigators across MGB and external collaborators.

This position provides an opportunity to gain hands-on experience with advanced genome-editing technologies in a collaborative core facility environment.

Essential Functions:

• Support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and lentiviral vector production workflows.
• Maintain and culture mammalian cell lines, including human iPSCs and primary cells, using aseptic technique.
• Perform routine molecular biology procedures, including DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and plasmid preparation.
• Support CRISPR-based genome-editing workflows, including reagent preparation, transfection/nucleofection, single-cell clone isolation and expansion, and genotyping of edited clones.
• Support next-generation sequencing (NGS) workflows, including amplicon/library preparation, sample quality control, and preparation of samples for sequencing.
• Perform cell characterization and quality-control assays, including immunofluorescence staining, microscopy, and other established assays.
• Maintain accurate experimental records, sample inventories, and project documentation according to established core SOPs.
• Perform routine data collection, organization, and basic analysis; communicate experimental results to core staff.
• Assist with laboratory inventory, reagent preparation, equipment maintenance, and general core operations.
• Follow established SOPs, biosafety requirements, and laboratory quality practices.
• Assist with troubleshooting and optimization of established gene-editing and molecular biology workflows under appropriate supervision.
• Participate in team meetings, training activities, and continuous workflow improvement.

Qualifications:

• Bachelor's degree required.
• Minimum of 1–2 years of hands-on research laboratory experience, including mammalian cell culture and molecular biology techniques.

Preferred

Competencies:

• Experience with human iPSC maintenance, passaging, cryopreservation, and/or differentiation.
• Familiarity with CRISPR/Cas9, base editing, prime editing, or related genome-editing technologies.
• Experience with transfection, nucleofection, and/or lentiviral vector production.
• Hands-on experience with NGS library preparation and basic sequencing QC.
• Experience with fluorescence microscopy, flow cytometry, or other cell-characterization techniques.
• Familiarity with basic sequence analysis and genome-editing analysis tools.
• Proficiency with data-analysis and documentation tools such as Excel, Graph Pad Prism, or Python/R.
• Previous experience in a core facility or other multi-project research environment.

Knowledge, Skills, and Abilities:

• Demonstrated ability to perform laboratory procedures accurately, consistently, and reproducibly.
• Strong understanding of aseptic technique and general laboratory safety practices.
• Strong organizational skills, attention to detail, and ability to manage multiple samples and tasks simultaneously.
• Ability to follow established protocols and accurately document experimental procedures and results.
• Ability to work effectively both independently and as part of a collaborative team.
• Effective written and verbal communication skills.

Additional Job Details (if applicable):

• This is an on-site role based at our lab site at 65 Landsdowne Street, Cambridge.
• Remote Type:
  Onsite
• Work Location:
  
  65 Landsdowne Street
• Scheduled Weekly
  
  Hours:
  
  40
• Employee Type:
  Regular
• Work Shift:
  
  Day (United States of America)
• Pay Range: $24.28 - $39.43/Hourly

EEO Statement:

0100 Mass General Brigham Incorporated is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.

To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran's Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at .