Associate Scientist

Quotient is seeking an Associate Scientist with experience in molecular biology, immunology and early drug discovery. This position will contribute to the translational validation of discovery platform targets in mechanistic and disease-relevant pharmacological models.

The ideal candidate will be a talented, goal-oriented and highly motivated individual who will play a vital role in progressing the company’s early discovery projects in inflammatory and autoimmune diseases. They will have a strong background in developing complex immune assays with primary human cells and tissues designed to understand the molecular basis of disease states as well as drug mechanism of action.

The candidate must be comfortable working in and contributing to a diverse, highly dynamic, fast-paced and cross-functional team environment.

• Use insights from literature and experimental results to design, optimize and implement robust in vitro experiments to validate target mechanisms and translational relevance to human disease
• Execute biochemical and cell-based assays using recombinant proteins, mammalian cell lines and primary cells to define drug mechanism of action
• Utilize creativity and critical thinking skills to innovate, troubleshoot/optimize experiments and to interpret data to drive go/no go decisions
• Must display attention to detail, sense of urgency and “outside the box” thinking
• Work under the supervision of senior members of the team and assist in designing and implementing research protocols
• Perform laboratory experiments and apply various laboratory techniques including compound screens, flow cytometry, ELISA, qPCR, Western blotting.
• Conduct in vitro and ex vivo assays, such as but not limited to over-expression, knockdown, knockout on immortalized and primary cells, stimulation, and cytokine profile or functional assays
• Collect, analyze and interpret scientific data accurately
• Maintain precise experimental records and documentation
• Contribute to the preparation of research articles, reports and presentations
• Follow lab safety procedures and rules to ensure a safe working environment
• Participate in regular team meetings to discuss progress, challenges, and future plans

• BS or MS in Immunology, Biochemistry, Molecular Biology, or a related discipline and 3+ years of industry experience for MS or 5+ years for BS
• In depth understanding of cellular and molecular biology techniques including mammalian cell culture, transfection, CRISPR, western blots, qPCR, immunofluorescence and flow cytometry
• Prior experience running cell-based compound screening assays
• Prior experience in an industrial setting, and familiarity with Good Laboratory Practice (GLP) as well as proficiency in the use of Electronic Laboratory Notebooks (ELN)
• Experience with experimental design and strong proficiency in assay execution, troubleshooting, data analysis, and data interpretation
• Outstanding organizational and record keeping skills
• Execution focused, team oriented, and highly motivated
• Proficiency with RNA-seq, cloning and image analysis
• Previous experience with lentiviral transductions or AAV transfection/transduction

• Encourage respectful disagreement and cultivate open-minded, ego-free interactions to continuously push each other towards excellence
• Seek out diverse perspectives; practice active listening and genuine curiosity to ensure all contributions are valued, regardless of source
• Recognize the impact of your behavior, language and attitudes, and strive for balanced, meaningful exchanges that enhance mutual growth and understanding in all interactions
• Use a company-first mindset to guide decision-making; prioritize team over individual success
• Take calculated risks and challenge convention…