UNIV - Research Specialist I - Department of Regenerative Medicine

Research Specialist I - Department of Regenerative Medicine

The Research Specialist I will work under general supervision by the PI and will be part of a basic science research laboratory that focuses on understanding cellular trafficking mechanisms in the gastrointestinal tract and liver. The position requires motivated, well-organized individuals with excellent communication skills.

The Research Specialist I will independently perform basic histological techniques including tissue processing, embedding and sectioning. The research specialist will be trained on immunohistochemical techniques and microscopy and will be expected to perform immunostaining and acquire micrographs. Additionally, the research technician will fix tissue and/or cells, embed tissue in paraffin or OCT and perform sectioning.

The Research Specialist I will work closely with the lab manager and perform mouse husbandry on 5-8 different transgenic and targeted mouse lines. The research specialist will learn mouse genotyping, weaning, mouse line maintenance and general husbandry. The research specialist will also aid in mouse dissection and tissue collection and processing.

The Research Specialist I will independently perform basic molecular biology techniques including DNA isolation, Polymerase chain reaction, agarose gel electrophoresis. The research specialist will extract RNA, synthesis cDNA, run qRT-PCR and perform cell culture experiments and maintenance. The research specialist will also generate gastrointestinal organoids from tissue and maintain intestinal organoids in vitro.

The Research Specialist I will perform basic lab maintenance responsibilities including glassware washing, solution making and laboratory organization. The research specialist will be responsible for making up solutions of PBS, keep track of stocks of essential items like gloves, reagents, antibodies. Help create an inventory of reagents and items in the lab. Maintain equipment such as scale and pipet accuracy.

The Research Specialist I will maintain accurate records of animals, and experimental procedures. Proficiency at programs including google-docs, excel, and word. The research specialist will assist in writing protocols, optimizing experiments, back up computers, perform statistical analysis and record experimental protocols and data.