UNIV - Research Specialist - Regenerative Medicine: Amy Engevik Lab

UNIV
- Research Specialist I
- Regenerative Medicine:
Amy Engevik Lab

Charleston, South Carolina

The research specialist I will work under general supervision by the PI and will be part of a basic science research laboratory that focuses on understanding cellular trafficking mechanisms in the gastrointestinal tract and liver. The position requires motivated, well-organized individuals with excellent communication skills.

Entity:
Medical University of South Carolina (MUSC
- Univ)

Worker Type:
Employee

Worker Sub-Type:
Research Grant

Cost Center: CC001062 COM REG MED Operations CC

Pay Rate Type:
Hourly

Pay Grade:
University-GEN
06

Pay Range: 37,200.

- 59,500.000

Scheduled Weekly

Hours:

40

Job Purpose:

The research specialist I will work under general supervision by the PI and will be part of a basic science research laboratory that focuses on understanding cellular trafficking mechanisms in the gastrointestinal tract and liver. The position requires motivated, well-organized individuals with excellent communication skills.

MUSC Minimum Training and Experience Requirements: A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience.

Preferred Qualifications:

A bachelor's degree in chemistry, biology or other life or health science is preferred.

Additional Knowledge, Skill and Ability:
Previous laboratory experience is considered helpful but not required.

Job Duties:

Perform basic lab maintenance responsibilities including glassware washing, solution making and laboratory organization. The research specialist will be responsible for making up solutions of PBS, keep track of stocks of essential items like gloves, reagents, antibodies. Help create an inventory of reagents and items in the lab. Maintain equipment such as scale and pipet accuracy. Essential 15%

Independently perform basic histological techniques including tissue processing, embedding and sectioning. The research specialist will be trained on immunohistochemical techniques and microscopy and will be expected to perform immunostaining and acquire micrographs. Additionally, the research technician will fix tissue and/or cells, embed tissue in paraffin or OCT and perform sectioning. Essential 35%

Work closely with the lab manager and perform mouse husbandry on 5-8 different transgenic and targeted mouse lines. The research specialist will learn mouse genotyping, weaning, mouse line maintenance and general husbandry. The research specialist will also aid in mouse dissection and tissue collection and processing. Essential 20%

Independently perform basic molecular biology techniques including DNA isolation, Polymerase chain reaction, agarose gel electrophoresis. The research specialist will extract RNA, synthesis cDNA, run qRT-PCR and perform cell culture experiments and maintenance. The research specialist will also generate gastrointestinal organoids from tissue and maintain intestinal organoids in vitro. Essential 20%

Maintain accurate records of animals, and experimental procedures. Proficiency at programs including google-docs, excel, and word. The research specialist will assist in writing protocols, optimizing experiments, back up computers, perform statistical analysis and record experimental protocols and data. Essential 10%

Minimum Requirements:

A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience.

Physical Requirements:

(

Note:

The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100°

F) when required due to…