UNIV - Research Specialist II - de Cubas Lab - Pharmacology & Immunology

Job Description Summary

The de Cubas Laboratory at the Hollings Cancer Center at Medical University of South Carolina is recruiting a Research Specialist I to contribute to cutting-edge research at the intersection of cancer biology and immunology. Our work focuses on understanding how genetic alterations such as SETD2 loss influence tumor immunogenicity and therapeutic response. By combining molecular biology, cell-based systems, and mouse models, we seek to translate mechanistic discoveries into strategies that improve cancer treatment.

The selected candidate will be responsible for the maintenance and logistical organization of a basic science laboratory, with particular emphasis on performing cell and molecular biology techniques, and ordering and maintaining lab supplies. We seek a highly motivated individual with previous experience in western blotting, RT-qPCR, and cell culture.

Medical University of South Carolina (MUSC - Univ)

Employee

Research Grant

CC001032 COM PHARM Administration CC

Hourly

University-GEN
08

45,300.

- 77,000.000

40

This Research Specialist II position will involve providing technical assistance to the Principal Investigator in cancer research studies, as well as assist in lab operations. Specific tasks will be as follows:

Cell culture and in vitro functional assays (30%). Maintaining cell lines; viability, migration, etc assay; transfection and infection.

Perform routine maintenance of and experimentation in mice (25%). These responsibilities include breeding and weaning, genotyping, and treatment with various agents. Assist with managing mouse colonies. Assists PI in ensuring that lab personnel adhere to IACUC animal protocol. Aid in IACUC inspections.

Assist with daily lab operations (20%). The selected candidate will assist with daily lab operations including stocking consumable items and reagents, making buffers, and aliquoting reagents. Facilitate general maintenance of the research space by working with MUSC to ensure that the laboratory area is maintained and that repairs, waste disposal, and cleaning needs are addressed in a timely manner.

Perform a number of molecular biology procedures (20%). Molecular biology experimentation employing various techniques, including isolation and purification of nucleic acids and proteins, PCR, Western blot, and plasmid cloning. Proteins will be further analyzed by immunoprecipitation followed by western blot or mass-spectroscopy. Chromatin or RNA immunoprecipitation reactions will be additionally prepared from isolated for downstream analyses.

Additional duties assigned by the Principal Investigator (5%). This will include helping prepare lab protocols, figures, and others.

A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area.

A bachelor's degree in biology or other life or health care science with biology research experience.

Must have previous experience in a laboratory.

Strong organizational, record keeping, interpersonal and communication skills required. Must be able to perform complex calculations, to follow complicated experimental procedures and methods and develop independence with tasks and experiments. Preference will be given to candidates with experience with animal husbandry, and colony management. Prior experience working with mice is valued, and recombinant DNA work, RNA and protein analysis are required.

Some weekend, holidays and/or evenings may be required.

Must be organized and have attention to detail. All personnel are expected to maintain laboratory notebook.

Minimum Requirements:

A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area.

Physical Requirements:

(

Note:

The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool.…