Postdoctoral Research Associate

Postdoctoral Research Associate

Texas A&M University is committed to enriching the learning and working environment by promoting a culture that respects all perspectives, talents & lived experiences. Embracing varying opinions and perspectives strengthens our core values which are:
Respect, Excellence, Leadership, Loyalty, Integrity, and Selfless Service.

The Texas A&M College of Veterinary Medicine & Biomedical Sciences (VMBS) is an institution that represents 100 years of growth from a small school of veterinary medicine in 1916 to its present role as a major veterinary educational, medical, and research center. Today, we are one of the largest schools of veterinary medicine in the United States, training nearly 600 Doctor of Veterinary Medicine students each year.

We support collaboration and teamwork that turns today's discoveries into proactive solutions for animals, humans, and the environment. Together, we strive to support the goals of the faculty, staff and students in teaching, research and outreach programs.

The Postdoctoral Research Associate, under general supervision, is responsible for conducting research on host–pathogen interactions for a USDA ARS collaborative project examining how prior exposure to moderately acidic conditions influences the ability of Salmonella to invade, survive, and replicate within bovine macrophages. The position combines bacteriology and mammalian cell culture with dual RNA-seq to characterize bacterial and host transcriptional responses across serotypes relevant to cattle and food safety.

The Postdoctoral Research Associate will design and perform infection and time-course experiments, generate and analyze host and bacterial transcriptomic data, and integrate phenotypic outcomes with differential gene expression to identify mechanisms of acid-adaptation–driven intracellular survival. The Postdoctoral Research Associate will maintain rigorous records, write and contribute to publications and sponsor progress reports, and may mentor student researchers. This position supports improved understanding of serotype-specific persistence in bovine systems and future strategies to reduce Salmonella risk in livestock populations.

Schedule:

This position may require work beyond normal office hours and/or on weekends to accommodate time-course experiments. This is an on-site position at the Texas A&M University campus and laboratory; it is not a remote or telework position.

Travel:
This position may require travel as required.

Please note:

This position is grant funded; future employment may be contingent upon future funding.

• Under general supervision, conducts research on host–pathogen interactions using a bovine macrophage infection model to determine how prior exposure to moderately acidic conditions influences Salmonella invasion, survival, and intracellular replication across serotypes relevant to cattle and food safety.
• Establishes and optimizes macrophage culture, density, viability, and infection conditions; validates the model using invasion- and replication-defective control strains and multiple multiplicities of infection (MOI).
• Prepares and standardizes acid-adapted and non-adapted bacterial cultures (turbidity standards, plate-based quantification); performs antimicrobial susceptibility (gentamicin protection) assays and confirms serotype purity.
• Performs infections and multi-timepoint sampling; quantifies association, invasion, and intracellular persistence via standardized lysis and plating; assesses macrophage counts, viability, and cytotoxicity for normalization.
• Extracts and purifies RNA from infected cells; evaluates RNA quality; performs ribosomal RNA depletion and strand-specific library preparation for dual host–pathogen RNA-seq.
• Maintains accurate records, analyzes research data, and summarizes results.

• Performs quality control and trimming of raw sequencing reads; aligns reads to host and bacterial reference genomes to characterize each transcriptome separately.
• Conducts dual RNA-seq differential expression analysis focused on stress-response pathways, virulence-associated genes, and inflammatory responses.
• Integrates phenotypic…