Research Associate II, Delivery

Position Summary

Ask Bio is seeking a motivated, detail-oriented, and collaborative Research Associate II to support AAV delivery research within the Delivery organization in R&D. This role provides hands‑on experimental support across molecular biology and cell‑based workflows that advance Ask Bio’s preclinical gene therapy pipeline. The Research Associate II will work closely with scientists within the Delivery team to execute established experimental protocols with increasing independence, generate high‑quality and reproducible data, and support day‑to‑day laboratory operations.

This position is based in Morrisville, North Carolina.

• Perform plasmid purification, genomic DNA extraction, RNA extraction, and PCR‑based assays (PCR, qPCR, ddPCR)
• Conduct routine mammalian cell culture activities, including maintenance of adherent cell lines and execution of cell‑based assays following established SOPs
• Perform in vitro assays including transfection and AAV transduction
• Support in vitro and in vivo studies through sample preparation, tissue processing, and downstream molecular analyses
• Accurately document experimental procedures and results in an electronic laboratory notebook in accordance with data integrity standards
• Analyze data, summarize results, and communicate findings through internal reports or presentations
• Maintain laboratory organization, including reagent preparation, inventory management, and inspection readiness
• Contribute to continuous improvement of laboratory workflows by following SOPs and escalating issues appropriately

• Bachelor’s degree in Molecular Biology, Biochemistry, Biotechnology, or a related scientific field with 2+ years of relevant laboratory experience; OR a Master’s degree in Molecular Biology, Biochemistry, Biotechnology, or a related field
• Hands‑on experience with molecular biology techniques, including DNA/RNA isolation, SDS‑PAGE, qPCR, ddPCR, and Western blot
• Prior experience with mammalian cell culture and aseptic technique
• Strong technical laboratory skills with the ability to independently execute protocols and experiments
• Ability to follow established protocols with minimal supervision and deliver reliable, reproducible results
• Strong organizational skills, attention to detail, and commitment to accurate documentation
• Ability to manage multiple tasks simultaneously and work effectively within a collaborative, cross‑functional team environment
• Proficiency with basic data analysis and common laboratory software tools (e.g., Excel, Graph Pad)

• Experience supporting AAV research or delivery programs, including familiarity with viral vectors or gene therapy concepts
• Exposure to AAV production or purification workflows at a research scale
• Experience preparing samples for downstream analyses such as NGS or other advanced molecular assays
• Comfort working across diverse experimental workflows and learning new techniques as project needs evolve

Ask Bio Inc. (Ask Bio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race, color, religion, gender, sexual orientation, gender identity, national origin, age, disability, veteran status or any other protected status prohibited under Federal, State or local laws. All employment decisions are based on valid job‑related requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website, you may request a reasonable accommodation to express interest in a specific opening by calling us at  or sending us an email at

Agencies:
Please do not contact any employee at Ask Bio about this requisition. Any resume submitted by a recruitment agency to any employee at Ask Bio, through any medium, will be deemed the sole property of Ask Bio unless the agency was engaged by Ask Bio Talent Acquisition team to recruit for that position. All agencies must have a prior executed service agreement with Ask Bio prior to any search engagement.

If a candidate who was submitted outside of the Ask Bio agency process is hired by Ask Bio, no fee or payment of any kind will be paid to the agency.