Sr. Research Associate, Delivery

Position Summary

Ask Bio Inc. is seeking a highly motivated, enthusiastic, and collaborative Senior Research Associate to support the development and discovery of next‑generation gene therapies utilizing state‑of‑the‑art non‑viral delivery technologies. The Senior Research Associate will work directly with the non‑viral delivery team to conduct experiments that advance our preclinical therapeutic pipeline.

The ideal candidate will have hands‑on experience with standard molecular biology techniques, including isolation of DNA, RNA, and protein from cells and tissues, and quantitative PCR (qPCR), RT‑PCR, ddPCR, ELISA, SDS‑PAGE, and Western blot. Prior experience with research‑grade lipid nanoparticle (LNP) formulation is preferred.

This position is based in Morrisville, North Carolina.

• Perform plasmid purification, genomic DNA extraction, RNA extraction, and PCR‑based assays (PCR, qPCR, ddPCR)
• Conduct small‑scale LNP formulation and analytical characterization for early research and preclinical in‑vitro and in‑vivo studies
• Perform in‑vitro cell‑based assays, including plasmid transfection, AAV transduction, LNP transfection, and primary cell culture
• Analyze data and effectively communicate results through formal reports and presentations for internal and external stakeholders
• Develop detailed experimental protocols and maintain an electronic laboratory notebook in accordance with good documentation and data integrity practices
• Maintain inventory and order reagents and materials to support timely project execution
• Ensure laboratory areas are clean, organized, and adequately stocked with necessary equipment and supplies

• Bachelor’s degree in molecular biology, biochemistry, or a related field with 5+ years of research experience; or a Master’s degree in molecular biology, biochemistry, or a related field with 2+ years of research experience
• Hands‑on experience with molecular biology techniques, including DNA/RNA isolation, SDS‑PAGE, qPCR, and Western blot
• Clear understanding of in‑vivo gene therapy approaches, including adeno‑associated virus (AAV) and lipid nanoparticles (LNP)
• Experience with cell line maintenance, including primary cell culture
• Strong technical laboratory skills with the ability to independently execute protocols and experiments
• Ability to manage multiple projects simultaneously and work independently in a fast‑paced, collaborative environment
• Ability to follow established molecular biology SOPs with minimal supervision
• Highly organized, self‑motivated, and able to work both independently and within cross‑functional teams
• Excellent written and verbal communication skills, including accurate documentation practices
• Proficiency in Microsoft Office Suite (Word, Excel, PowerPoint)
• Strong problem‑solving skills and sound scientific judgment

• Experience with research‑grade LNP formulation and analytical characterization
• Experience with next‑generation sequencing (NGS)
• Understanding of statistics and data analysis/interpretation
• Experience using Snap Gene and Graph Pad

Ask Bio Inc. (Ask Bio) is an Equal Opportunity Employer and does not discriminate against any employee or applicant for employment because of race, color, religion, gender, sexual orientation, gender identity, national origin, age, disability, veteran status or any other protected status prohibited under Federal, State or local laws. All employment decisions are based on valid job‑related requirements. If you are a qualified individual with a disability or a disabled veteran and are unable or limited in your ability to use or access our website, you may request a reasonable accommodation by calling us at (919) 561‑6210 or sending us an email at