Research Associate II, Product Development

Ask Bio Inc., a wholly owned and independently operated subsidiary of Bayer AG, is a fully integrated gene therapy company dedicated to developing life-saving medicines and changing lives. The company maintains a portfolio of clinical programs across a range of neuromuscular, central nervous system, cardiovascular, and metabolic disease indications with a clinical-stage pipeline that includes investigational therapeutics for congestive heart failure, limb-girdle muscular dystrophy, multiple system atrophy, Parkinson’s disease, and Pompe disease.

Ask Bio’s gene therapy platform includes Pro
10, an industry-leading proprietary cell line manufacturing process, and an extensive array of capsids and promoters. With global headquarters in Research Triangle Park, North Carolina, and European headquarters in Edinburgh, Scotland, the company has generated hundreds of proprietary capsids and promoters, several of which have entered pre-clinical and clinical testing.

Our vision:
Pioneering science to create transformative molecular medicines.

Our mission:
Lead innovative science and drive clinical outcomes to transform people's lives.

Our principles:

Advance innovative science by pushing boundaries.

Bring transformative therapeutics to patients in need.

Provide an environment for employees to reach their fullest potential.

Our values:

Be a Pioneer. We are not afraid of the impossible and to innovate to make gene therapies accessible to those in need.

Cultivate Collaboration. Strive to be the best teammate, actively listen, openly communicate, and embrace diverse points of view.

Embrace Responsibility. We are humbled by the enormity of our mission. We hold a relentless commitment to advance science and clinical outcomes for our patients, families, and caregivers.

Raise the Bar. Continuously drive improvements and efficiencies. Seek and provide constructive feedback. Have a bias for learning and action.

Act with Uncompromising Integrity. Be honest, transparent, and committed to doing what’s right in every situation. Make clear commitments and follow through.

Position Summary The Research Associate II is a technical role utilizing a broad range of experimental techniques to support platform, therapeutic pipelines, and R&D activities, with a primary focus on early cardiovascular programs. The RA II will perform optimization, development, and data delivery with increased independence, supporting key preclinical studies in gene therapy R&D.The ideal candidate will have:

Direct expertise with mammalian cell culture, including primary and immortalized cell systems, and strong aseptic technique

Direct experience with quantitative molecular biology techniques, including isolation of DNA, RNA, and protein from cells and tissues; quantitative PCR and RT‑PCR (preference for direct experience with ddPCR); ELISA; SDS‑PAGE; and Western blot/automated Western (e.g., Jess)
Strong data collection, documentation, and reporting skills, with the ability to work independently and as part of a research team

This position is office-based based in RTP, NC and reports to the Senior Scientist, Product Development.

Job Responsibilities Support scientists in the design, planning, and execution of experiments to analyze protein, RNA, and DNA from in vitro and in vivo studies, contributing to key studies in gene therapy R&DPerform a broad range of experimental techniques independently (e.g., cell culture, nucleic acid and protein extraction, qPCR/RT-qPCR/ddPCR, ELISA, SDS-PAGE, Western blot/Jess, and related assays) to support platform and therapeutic programs

Process, track, and analyze large numbers of samples in parallel while maintaining high quality and consistency in assay execution

Maintain accurate, up to date records of all…