Postdoctoral Research Fellow - Pavani Lab

SHIFT:
Day (United States of America)

Seeking Breakthrough Makers

Children’s Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.

At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care—and your career.

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The Pavani Lab at The Children’s Hospital of Philadelphia (CHOP) seeks a motivated postdoctoral fellow to join a collaborative, multidisciplinary team focused on genome editing and red cell engineering for translational research. The successful candidate will lead projects that apply CRISPR/Cas9 and other genome engineering approaches in mammalian cell systems, with the goal of advancing novel therapeutic strategies and improving blood transfusions.

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• Design, optimize, and execute genome‑editing experiments (CRISPR/Cas systems, base editors, prime editors, or equivalent) in mammalian cells.
• Develop and maintain cell cultures, including induced pluripotent stem cells (iPSCs) and immortalized lines.
• Perform genome engineering workflows: vector design and cloning, transfection/electroporation, editing efficiency and genotyping assays.
• Characterize edited cells using flow cytometry (including sorting), immunophenotyping, and functional assays.
• Prepare next‑generation sequencing (NGS) assays for on‑/off‑target analysis and deep sequencing of edited loci; analyze sequencing data.
• Troubleshoot experiments, optimize protocols, and maintain rigorous documentation and lab records.
• Present data at internal meetings and conferences; contribute to manuscript and grant preparation.

• PhD in Molecular Biology, Genetics, Cell Biology, Bioengineering, or a related field.
• Demonstrated hands‑on experience with genome editing techniques (CRISPR/Cas or similar).
• Strong experience in mammalian cell culture and experimental manipulation.
• Practical experience with flow cytometry (sample prep, acquisition, and analysis).
• Ability to design experiments, analyze data quantitatively, and communicate findings clearly.

• Experience with next‑generation sequencing workflows relevant to genome editing (amplicon‑seq, targeted sequencing, library prep) and basic bioinformatic analysis of NGS data.
• Previous work with induced pluripotent stem cells (iPSC) — culture, maintenance, differentiation, or genetic manipulation.
• Familiarity with erythropoiesis and flow cytometry.
• Experience in vector design, and safe handling of viral vectors (Lenti).
• Track record of publications in the genome engineering or cell therapy fields.
• Strong organizational skills, ability to work collaboratively across diverse teams, and mentoring experience with junior trainees.

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient‑facing responsibilities must receive an annual influenza vaccine.

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$20,800.00 - $ Annually

Salary ranges are shown for full‑time jobs. If you’re working part‑time, your pay will be adjusted accordingly.

If you are applying for a Research Post‑Doctoral Fellow role, please refer to this link for salary guidelines (copy & paste into your browser): https://-3

At CHOP, we are committed to fair and transparent pay practices. Factors such as skills and experience could result in an offer above the salary range noted in this job posting.  for more information regarding CHOP’s Compensation and Benefits.