Molecular Biology Lead Technical Support

Eagle Health is seeking a Molecular Biology Lead Technical Support candidate to join our team in Fort Detrick, MD.

The employee shall provide laboratory support for research efforts within the Center for Genome Sciences in the Molecular Biology Division. Enrollment in the BPRP (BSL-3 and BSL-4) is required. Employee will conduct basic and applied research on current and emerging threats in support of the biodefense and infectious disease program. The employee shall aid in the execution of scientific studies including extraction of nucleic acids from different biological matrices, quantification and quality of RNA and DNA, PCR amplification, sequencing library preparation and quantification, high-throughput screening assays and/or high-throughput sequencing on infectious agents.

The employee will support processing of tissues from animal, data analyzing and maintaining laboratory areas and equipment. The employee shall provide supervisory support to junior contract personnel, training to new personnel, and perform routine laboratory safety inspections for assigned areas.

• The employee will perform work on automation equipment that can handle and process high numbers of samples such as Hamilton STAR and King Fisher liquid handlers. Perform purification of nucleic acids from inactivated viral cultures, animal tissues and human subject blood. Assist with design and performing nucleic acid-based assays for tissue culture and/or animal tissue samples.
• The employee will perform research in next generation sequencing (NGS) including Illumina (iSeq, Mini Seq, MiSeq, Next Seq 2000 and Nova Seq 6000) and Oxford Nanopore Technologies (MinION and GridION). Will also perform nucleic acid extraction, bioanalysis, and quantitation, conventional PCR and qPCR, manual and automated library preparation including RNAseq and DNAseq, and DNA size selection and fractionation including SAGE ELF and Blue Pippin.
  
  Additionally, will perform molecular biology work including cloning, primer design, Gibson Assembly, restriction enzyme digestion, ligation, site directed mutagenesis, in vitro transcription, and reverse transcription.
• The employee will operate and analyze data collected on Western Blot equipment such as Protein Simple WES, DNA fragmentation using Covaris systems, Real-Time PCR Detection System such as Bio Rad CFX, and quantification and QC of RNA and DNA using Nano Drop 8000, Qubit 3.0 Fluorometer, and Agilent 2200 and 4200 Tape Station systems. Will maintain mammalian cell cultures including primary and secondary cell lines and cell line cultures in BSL-2, BSL-3 and BSL-4 as required.
• The employee will evaluate effects of pathogen infection on host (animal models or in vitro cultures) analyzing gene expression using RNAseq protocols (bulk, targeted, single cell or spatial) to identify deregulated signaling pathways that can lead to novel treatment strategies for infectious diseases. Will also evaluate the pathogen by population genomics and whole genome sequencing.
• The employee will support animal studies when required by assisting the study personnel to collect and process animal specimens. Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays.
• The employee will train new employees, ensure SOPs are followed, deviations are recorded, and safety procedures are observed.
• The employee will assist in the preparation of technical reports and manuscripts relating to their research efforts. Employee will present results in written and oral format publicly and internally as requested.
• The employee will write and review standard operating procedures (SOPs), capture forms, and all supporting documentation in accordance with Good Laboratory Practice (GLP) and Well-Documented (WD) study regulations as required.
• The employee will be responsible for assessing and maintaining laboratory supplies. Will inventory supplies and ensure supply orders are coordinated with Division resources. Will ensure that all assigned laboratory space is orderly and compliant with all regulatory guidance.
• Employee will communicate to the PI or assigned TPOC…