Scientist Cell Line Development Group

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Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Scientist In The Cell Line Development Group to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Allergy and Infectious Diseases (NIAID) located in Gaithersburg, MD.

Benefits We Offer:

• 100% Medical, Dental & Vision Coverage for Employees
• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Position Overview

Certifications & Licenses

• Master's or Ph.D. in Biological Sciences or a related field with a minimum of 2 years of experience in an industrial setting focused on the development of stable cell lines for vaccine and biotherapeutic candidates

Field of Study

• Biology

Software

• MS Office Suite

Skills

• Extensive hands-on experience with aseptic technique and mammalian cell culture for the purpose of generating clonally derived cell lines to produce monoclonal antibodies or vaccine candidates

• Demonstrated ability to produce and screen hundreds of clonally derived CHO or 293 cell lines for top performers

• Expertise in designing and constructing plasmids for gene expression using molecular cloning methods

• Hands-on experience with multiple supporting technologies for cell line engineering, including cell counting, high-throughput transfection, single-cell cloning/deposition, flow cytometry, automated liquid handling systems, and automated small-scale bioreactor systems

• Ability to independently design experiments and work plans and manage project timelines for multiple complex projects simultaneously

• Experience preparing technical reports, SOPs, data presentations, and documents needed for technology transfer and regulatory filing

• Expertise in molecular biology techniques for genetic characterization of cell lines, including RT-PCR and NGS

Deliverables

• Inspect Equipment — Ad-Hoc

• Meet with lab members to present updates — Ad-Hoc

Statement of Work Details

• Plan and execute the generation of stable cell lines for preclinical and clinical material production to advance candidates including monoclonal antibodies, recombinant proteins, and virus like particles — 1

• Use molecular biology techniques, including RT-PCR and NGS, for genetic characterization of cell lines — 2

• Gene synthesis including oligo synthesis, plasmid design, and construction — 3

• Independently execute all steps of the process including plasmid construction, stable pool generation, single cell cloning, and top clone selection

• Supports the planning and execution of research experiments in the context of the laboratory's overall research program

• Draft and verify data for CMC sections of pre-IND and IND documents to ensure successful registration of candidates

• Design, perform, and troubleshoot experiments and analyze data for both development and deliverable projects

• Supports laboratory staff on research projects and techniques

• Maintain and support one or more pieces of laboratory instrumentation

• Maintains laboratory equipment and the training of others in their use

• Assists in maintaining…