Ingénieur-e d'études en biologie végétale

Job Title

Your tasks in a nutshell

You will work at the Laboratory of Plant Microbe Environment Interactions (LIPME) in Castanet Tolosan as part of a collaboration between the teams "Symbiotic Functions, Genomes and Evolution of Rhizobia" (SYMEVOL) and "Endosymbiotic Infection and Nodule Development" (ENOD). Our teams are interested in the infection stages of legumes by rhizobia using two model systems, the Medicago truncatula/Sinorhizobium meliloti and Mimosa pudica/Cupriavidus taiwanensis interactions.

Your work will be integrated into an ANR project whose objective is to study the role of plant polarity proteins in the establishment of these symbioses. One of your tasks will be to optimize the transformation protocol of Mimosa pudica by Agrobacterium rhizogenes. You will then analyze the spatio-temporal expression of Mimosa pudica polarity proteins using transcriptional (Promoter-reporter GUS fusions) and translational (fluorescent fusion protein) fusions.

You will also determine the effect of constructs disrupting the activity of polarity proteins on the formation and infection of nodules and compare the results with those obtained on Medicago. You will use plant in vitro culture techniques, Agrobacterium rhizogenes transformation, molecular biology (plasmid construction, PCR) and classical and confocal microscopy. You will carry out this work in collaboration with a post-doctoral student from the ENOD team and in consultation with the other partners of the project.

Recommended education:
Master

Desired knowledge:
Plant biology (in vitro cultures, transformation), molecular biology, basic microbiology

Required skills:

motivation, scientific rigor, interest in plant-microorganism interactions, teamwork.

• Level Level 6 License/equivalent diplomas