Research Technologist- iPSC Cell Culture and Genome Engineering

This position is funded for 12 months; continuation past 12 months will be based on university need, performance, and/or availability of funding.

We are seeking a highly motivated Research Technologist to join a cardiovascular research laboratory focused on human induced pluripotent stem cell (iPSC) models of cardiac diseases inherited. The successful candidate will play a key role in establishing and maintaining iPSC cultures. This position offers an excellent opportunity to gain hands-on experience with cutting-edge stem cell technologies, genome engineering, and translational cardiovascular research in a collaborative academic environment.

• Perform routine maintenance, expansion, cryopreservation, and quality control of human iPSC lines.
• Differentiate iPSCs into cardiomyocytes and other relevant cell types following established protocols.
• Isolate and expand single-cell clones and perform molecular validation using PCR, Sanger sequencing, and related techniques.
• Conduct molecular and cellular assays including DNA/RNA extraction, qPCR, Western blotting, immunofluorescence staining, and flow cytometry as needed.
• Maintain accurate laboratory records, experimental documentation, and electronic databases.
• Prepare reagents, maintain cell culture supplies, and ensure compliance with laboratory safety procedures.
• Assist in data analysis, figure preparation, and presentation of research findings.
• Participate in laboratory meetings and contribute to collaborative research projects.

• Experience with mammalian cell culture.
• Strong organizational skills and attention to detail.
• Excellent written and verbal communication skills.
• Ability to work independently while contributing effectively within a collaborative research team.

• Bachelor's or master's degree in Cell Biology, Molecular Biology, Genetics, Biomedical Sciences, Biotechnology, or a related field.
• Experience culturing human iPSCs or embryonic stem cells.
• Experience with stem cell differentiation, particularly cardiac differentiation.
• Familiarity with PCR, cloning, DNA sequencing, and molecular biology techniques.
• Experience with fluorescence microscopy, flow cytometry, or next-generation sequencing is desirable.
• Prior experience in cardiovascular, stem cell, or genetic disease research.

• Strong motivation to learn new techniques.
• Excellent problem-solving and troubleshooting skills.
• Ability to manage multiple projects simultaneously.
• Commitment to maintaining rigorous scientific standards and reproducible research.
• Interest in translational medicine and cardiovascular genetics.

Our laboratory investigates the molecular mechanisms underlying inherited cardiac diseases using patient-derived iPSC models, CRISPR-based genome engineering, advanced molecular biology, and functional genomic approaches. The successful candidate will work closely with trainees and collaborators while receiving mentorship in cutting-edge stem cell and genome editing technologies.

This position provides outstanding preparation for graduate school, medical school, physician-scientist training, or careers in biotechnology and the pharmaceutical industry.

Bachelor's Degree1+ years of relevant experience; or an equivalent combination of education and experience accepted

Required

Certifications:

None

Employment with the University will require successful completion of background check(s) in accordance with University policies. Penn State does not sponsor or take over…