DOH-NGS Specialist

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IN-DOH-NGS Specialist

550 W 16th St Ste B, Marion County, Indianapolis, IN 46202

Long Term

State of IN

Position requires NGS (Next Generation Sequencing) experience using an Illumina Instrument or a PacBIO instrument. Position requires performing tests on potentially dangerous specimens. Position requires contractor to utilize all personal protective equipment in the designated lab area. Position is Monday through Friday, 7.5H days, 37.5H week; core hours are 8:15am 4:45pm (one hour lunch) but schedule can be flexible, with the possibility of a 30 minute lunch.

Weekends would not be required. This position is part of the Indiana Department of Health Laboratory, Advanced Molecular Detection Division.

Incumbent is responsible for coordinating and performing the full range of molecular biological examinations and analyses, recording, interpreting, and result reporting of tests on specimens which may include human body fluids, tissues (human and animal), isolates from clinical specimens, and environmental samples to the submitting physician, clinic, hospital, or public health agency. Incumbent is also responsible for the development, validation, and implementation of new molecular tests, preparation of validation documents and SOPs, and troubleshooting of the existing molecular tests.

Special emphasis for this position will be around genomic sequencing, maintenance of instrumentation for sequencing, and troubleshooting sequencing assays.

• Extraction of nucleic acids from specimens, isolates, and samples via a variety of manual and automated methods.
• Utilize molecular techniques such as PCR, MALDI-TOF, and NGS.
• Participate in the evaluation, development, validation, and implementation of new testing methods used in clinical/reference laboratory work, as well as preparation of validation documents and SOPs.
• Provide technical oversight of the daily performance of high complexity testing; strictly follow the laboratory's procedures for specimen handling and processing, test analyses, reporting, and maintaining records of test results.
• Maintain laboratory record system of submitted samples and specimens and their possible referral.
• Evaluate, interpret, validate laboratory results, and report findings.
• Conduct quality control checks on laboratory methods, materials, and equipment.
• Document all quality control activities, instrument calibrations, and maintenance performed.
• Participate in evaluation of new products used in clinical/reference laboratory work.
• Perform all related work as required.

• Extensive knowledge of the principles, theories and practices of molecular biology, and related sub-specialties.
• Experience with Next Generation Sequencing platforms, technologies, and procedures.
• Specialized knowledge of current scientific methods and testing procedures and the ability to apply them when seeking solutions to public health laboratory problems.
• Specialized knowledge of and ability to use a full range of standard technical equipment, complex scientific apparatus, and automated techniques of analysis.
• Extensive knowledge of laboratory safety practices and principles.
• Aware of state and federal laws, rules, regulations, and policies concerning the program area (i.e. CLIA).
• Ability to meet requirements for personnel certification as a technologist pursuant to CLIA.
• Ability to effectively communicate technical information both verbally and in writing and maintain productive working relationships.
• Ability to satisfactorily participate in proficiency testing programs and recognize QA problems.
• Ability to compile, analyze, evaluate, and prepare laboratory reports.
• 2 years of high complexity laboratory experience as testing personnel, with experience providing oversight of daily performance of high complexity testing.

N/A

Incumbent is engaged in scientific analyses that demand accuracy and demonstrated proficiency in applying laboratory analytical skills to select pertinent guidelines and formulated plans for problem solving. Projects involve many complex variables of great intricacy and the microbiologist is often called upon to analyze and identify unusual specimens/samples. Work requires considerable use of analytical judgement and technical knowledge in order to draw accurate, logical conclusions.

Incumbent will also be required to develop and research new testing procedures.

Incumbent works independently as an expert in the Biothreat laboratory. Technical and/or detailed instruction is provided only upon request when a procedural change and/or special project necessitates it. Incumbent maintains established safety practices when working with materials that have a chemical hazard or…