Research Assistant I - Laboratory of Dr. Ron Evans

Research Assistant I

The Research Assistant I will support NIH R01-funded research focused on human induced pluripotent stem cell (iPSC) differentiation and beta cell transplantation for the development of novel cell replacement therapies for diabetes. Working under the direction of the Principal Investigator and in collaboration with postdoctoral fellows, staff scientists, and research collaborators, the incumbent will assist with maintaining human iPSC cultures, performing multistage pancreatic and beta cell differentiation protocols, characterizing differentiated cells, and supporting transplantation studies in mouse models of diabetes.

The successful candidate will be responsible for following established laboratory protocols, maintaining accurate and detailed experimental records, and ensuring consistent application of aseptic techniques and quality standards. This position requires strong organizational skills, attention to detail, and the ability to execute complex, time-sensitive procedures with accuracy and reproducibility. The Research Assistant I will have the opportunity to develop technical expertise in stem cell biology, molecular and cellular biology techniques, genome engineering, and small-animal research while contributing to a collaborative and multidisciplinary research environment.

The Salk Institute is an internationally renowned research institution that values all members of our scientific community. We seek bold and interactive leaders passionate about exploring new frontiers in science. Our collaborative community embraces perspectives across discipline, professional acumen, and unique life experiences, fostering innovation, and a sense of belonging. Together, we strive to improve the wellbeing of humanity through groundbreaking research.

Founded by Jonas Salk, developer of the first safe and effective polio vaccine, the Institute is an independent, nonprofit research organization and architectural landmark: small by choice, intimate by nature, and fearless in the face of any challenge. Salk's vibrant community has many talented individuals from varied backgrounds, each playing a crucial role in driving our mission forward. From visionary leaders to dedicated administrators and brilliant faculty members, the Institute is united by a shared passion for scientific exploration and innovation.

Maintain, expand, passage, cryopreserve, and recover human iPSC lines using sterile technique.

Prepare reagents and support multistage differentiation of iPSCs into pancreatic progenitors, endocrine progenitors, and beta cell or islet-like organoids.

Monitor cell culture quality, differentiation progress, and maintain accurate experimental records.

Assist with banking, quality control, and characterization of genetically engineered iPSC lines.

Perform DNA, RNA, and protein extraction and assist with molecular and cellular assays, including PCR, RT-qPCR, immunoblotting, ELISA, immunofluorescence, flow cytometry, and microscopy.

Support analysis of beta cell differentiation and function through marker expression, viability assays, insulin/C-peptide measurements, and glucose-stimulated insulin secretion assays.

Maintain accurate sample records, inventories, and experimental data.

Assist with preparation and transplantation of iPSC-derived beta cell clusters or islet-like organoids under approved animal protocols.

Assist with animal handling, monitoring, dosing, blood glucose measurements, and sample collection following appropriate training.

Support tissue collection, processing, and analysis while maintaining required animal and experimental records.

Prepare media, buffers, solutions, and reagents; maintain laboratory supplies, equipment, and inventory.

Assist with ordering, receiving, labeling, and storage of laboratory materials.

Follow laboratory safety procedures and participate in meetings, data discussions, and troubleshooting activities.

Performs other related duties as assigned by management.

This job has no supervisory responsibilities.

No professional experience required.

Education:

Bachelor's degree in Biological Sciences or related scientific discipline required. A minimum of 2 years of…