Cell Line Development; CLD) Scientist

Contract Cell Line Development (CLD) Scientist

Lexington, Massachusetts, United States

At Keros, our mission is to deliver significant clinical benefit to a wide range of patients with disorders that are linked to dysfunctional signaling of the transforming growth factor-beta (TGF-ß) family of proteins. With a focus on developing differentiated product candidates that are designed to alter TGF-β signaling, we aim to target the pathways critical for the growth, repair and maintenance of blood cells and a number of tissues, including bone, skeletal muscle, adipose and heart tissue.

We believe our product candidates have the potential to unlock the full therapeutic benefits of modulating the TGF-β superfamily and provide disease-modifying benefit to patients.

Job Summary

Keros Therapeutics is seeking a highly motivated and collaborative individual to join our protein biochemistry group within our Research Department. Our group exploits multidisciplinary approaches in protein sciences and protein engineering, to better the understanding of the biochemical, biophysical, and structural functional properties of the biomolecules within the TGF-β superfamily that drive the signaling pathway. Our efforts greatly contribute to advance innovative research and drug discovery and heavily support our discovery pipeline.

Primary Responsibilities

• Perform mammalian cell line development activities, including transfection, selection, minipool and clone generation
• Establish efficient and scalable workflows for screening and evaluating cell lines and clones, troubleshooting as needed.
• Develop, optimize and establish high-producing stable cell lines of potential new drug candidates such as recombinant proteins and antibodies
• Design and execute experiments to evaluate cell growth, productivity, stability, metabolic profiles, and product quality
• Contribute to routine mammalian expression of our therapeutic molecules through stable expression in CHO cells to support ongoing studies, as needed
• Perform assays and characterization techniques (SDS-PAGE and SEC-HPLC) to characterize R&D molecules
• Maintain clear, accurate documentation of computational and experimental laboratory work and summarize results as needed in study reports and contribute to documentation of cell line development protocols and workflows.
• Communicate progress, results, and challenges effectively.
• Provide updates at research meetings in both oral and written formats.

Qualifications:

• A bachelor's or master's degree in biochemistry, chemistry, engineering or a related field with 8+ (Bachelor's) or 5+ (Master's) years of industrial experience in cell line development
• Extensive hands-on experience with protein expression in mammalian cells is required
• Strong hands-on experience with CHO cell line development activities is required.
• Experience with high-throughput cell line development and/or clone screening is highly desirable.
• Experience with mammalian expression in wave bioreactors and/or stirred tank bioreactors is highly desirable.
• Familiarity with protein characterization techniques is a plus.
• Experience working with wet lab benchwork, lab etiquette, pipetting, and volumetric handling. Ability to perform chemical stoichiometric calculations and buffer preparation.
• Ability to work as part of a cross-functional team, and independently with supervision.
• Exhibit excellent communication and organization skills, detail-oriented, proficient in MS office, data analysis, and slide presentation.
• Displays a high degree of maturity, honesty, trust, accountability and integrity. Consider a scientific problem/question as an opportunity for innovation and intellectual growth.

This pay range represents the base hourly rate or base annual full-time salary for all positions in the job grade within which this position falls. The actual base salary offer will depend on a variety of factors including experience, education, and other relevant factors.

Potential base pay range

$95 - $111 USD

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