Scientist, Research Scientist

About Boundless Dx

Boundless Dx is a diagnostics technology company developing next‑generation platforms that deliver rapid, actionable insight in real‑world environments. Focused on rethinking how diagnostic information is generated, interpreted, and applied, we are building systems that enable faster, more confident decision‑making across a range of use cases.

As we move toward commercialization, Boundless Dx is bringing together science, engineering, and data to solve complex problems at the intersection of technology and practical application. We are building a team that thrives on innovation, cross‑disciplinary collaboration, and the opportunity to shape both the product and the company during a pivotal stage of growth.

As a Scientist / Associate Scientist on the Protein Purification team, you will work within Boundless Dx’s research and development group to purify, concentrate, and characterize the antibodies and protein antigens that form the core of Boundless Dx’s diagnostic product range. You will independently execute purification workflows on FPLC/AKTA platforms, develop and pack chromatography columns, immobilize proteins onto solid matrices, and process moderate volumes of material while supporting scale‑up for commercial production.

Working under the guidance of senior scientists, you will own your assigned purification tasks end‑to‑end, document results to quality standards, and present your data in project update meetings.

• Purify antibodies from blood‑derived materials (e.g., serum, plasma, ascites, and other blood fractions) as well as recombinant antibodies and protein antigens, using established and newly developed methods.
• Operate, maintain, and troubleshoot FPLC/AKTA chromatography platforms (e.g., ÄKTA pure/avant/start), including method building, run execution, and data analysis.
• Apply a broad range of purification and concentration methodology, including affinity chromatography, ion exchange (CEX/AEX), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC), alongside ultrafiltration/diafiltration (TFF), buffer exchange, and precipitation.
• Prepare, pack, and qualify chromatography columns, select and screen resins, and verify column performance (e.g., HETP/asymmetry) prior to use.
• Cross‑link and immobilize proteins onto solid matrices and resins to generate custom affinity supports and conjugates, optimizing coupling chemistry, ligand density, and yield.
• Process moderate volumes and support scale‑up and tech transfer of purification processes from bench through to reproducible, cost‑effective commercial production, potentially including support of contract manufacturing partners.
• Develop concentration, formulation, and stabilization methods, assessing protein solubility, aggregation, and stability across buffer/formulation conditions to maximize performance and shelf life.
• Characterize and QC purified materials, evaluating antibody behavior and performance and running binding/functional assays such as Octet (BLI), ELISA, and lateral flow, together with purity and identity testing (e.g., SDS‑PAGE, SEC‑HPLC, A280, endotoxin).
• Plan and execute experiments in a logical, well‑controlled manner, perform rapid troubleshooting, and analyze data to inform purification strategy and process improvements.
• Maintain accurate, timely documentation in accordance with Boundless Dx’s Design Control process and quality management system, including batch/lot records, method protocols, and laboratory notebooks.
• Perform quality‑control activities on purified lots, including incoming raw material checks, lot‑to‑lot comparison, and escalation of out‑of‑specification results.
• Manage materials and inventory and support reagent and supplier coordination.
• Contribute to lateral flow assay development and the integration of purified reagents into point‑of‑care products.
• Report and summarize results in project update meetings, communicating progress, risks, and timelines to the team and senior stakeholders.

Strong hands‑on laboratory skills with sound understanding of protein chemistry, chromatography…