UNIV - Staff Scientist - Department of Regenerative Medicine

UNIV
- Staff Scientist
- Department of Regenerative Medicine

MUSC Health |

MUSC Health | Posted Mar 2

Full-time

Charleston

Negotiable

Unknown

Under moderate general supervision, will design, execute, evaluate and communicate results of scientific experiments, prepare figures, and participate in scholarly publications. Also, manage and coordinate activities of a basic science laboratory, develop and troubleshoot protocols, perform complex molecular biology and cell biology assays, implement lab safety protocols, guide and mentor students, and assist the PI with generating preliminary data for grant submissions.

PhD degree in chemistry, biology, or other natural, life or health care science and 2-year work experience directly related to the assigned research area.

• 30% - Develop own projects, under moderate guidance by the PI. Plan, coordinate and communicate project goals, timelines and budgeting. Organize, analyze data and present data in manuscript figures, lab meetings and departmental seminars. Contribute to scholarly publications. Advise the PI on own and all other projects in the lab. Assist the PI in preparation of manuscripts, seminars and by generating preliminary data for grant proposals.
• 20% - Perform project-appropriate mouse colony maintenance, mouse tissue biopsy and animal husbandry. Perform phenotypic examination at the organ, tissue, and cellular levels.
• 20% - Examine and analyze tissues from various organs for DNA, RNA, and/or protein markers. Perform histology and subsequent immunofluorescence, immunohistochemistry and related imaging of mouse tissues.
• 10% - Perform standard cell/tissue culture and cell-based assays (cell line maintenance and storage, transfection, etc). Perform and develop protocols for standard and complex molecular biology techniques (DNA/RNA isolation, PCR, qRT-PCR). Assist with analyzing of RNA sequencing data. Perform protein isolation from cells/tissues, immunoprecipitation, and Western blotting.
• 10% - Prepare solutions, reagents and glassware/equipment as necessary for project(s) needs. Ensure recording of experimental results and recording and maintenance of laboratory procedures, protocols and databases. Troubleshoot protocols and experimental procedures and advise the PI and other lab members on this and on different projects in the lab.
• 5% - Train and supervise students, volunteers and trainees in the proper application of techniques, protocols, equipment handling, and safety procedures in the lab. Supervise enforcement of chemical and biological laboratory safety procedures and handling of hazardous materials by all members in the lab.
• 5% - Maintain inventory of lab supplies, perform ordering of supplies and monitor budget and spending.

Ability to perform job functions in an upright position. Ability to perform job functions in a seated position. Ability to perform job functions while walking/mobile. Ability to climb stairs. Ability to work indoors. Ability to work in all cold temperature extremes, i.e. spot coolers. Ability to bend at the waist. Ability to twist at the waist. Ability to perform 'pinching' operations.

Ability to fully use both hands/arms. Ability to perform repetitive motions with hands/wrists/elbows and shoulders. Ability to fully use both legs. Ability to reach in all directions. Possess good finger dexterity. Ability to maintain tactile sensory functions. Ability to maintain good olfactory sensory function. Ability to lift and carry 30 lbs., unassisted. Ability to lift objects, up to 30 lbs.,

from floor level to height of 72 inches, unassisted. Ability to lower objects, up to 30 lbs., from height of 72 inches to floor level, unassisted. Ability to push/pull objects, up to 50 lbs., unassisted. Ability to lift and carry objects up to 30 lbs. a distance of five (5) floors and to outlying buildings. Ability to maintain 20/40 vision, corrected.

Ability to see and recognize objects close lity to see and recognize objects at a distance. Ability to match or discriminate between colors. Ability to determine distance/relationship between objects; depth perception. Good peripheral vision capabilities. Ability to maintain hearing acuity, with correction. Ability to perform gross motor functions with frequent fine motor movements. Ability to be qualified physically (by medical personnel) for respirator use, initially and annually.

Ability to work alone in isolated areas, such as darkrooms. Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required.

The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All…