Assistant Scientist-Proteomics

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The University of Miami/UHealth Department of SCCC has an exciting opportunity for a Full Time Assistant Scientist to work at the UHealth campus in Miami, Fl.

CORE JOB SUMMARY
The Assistant Scientist carries out a particular research agenda in support of the University's research goals and objectives. Moreover, the Assistant Scientist, SOM contributes to the scientific community by publishing
research findings and promotes the University's reputation as a leader in the field.

CORE JOB FUNCTIONS

• Contributes to basic and applied research activities and authors scientific publications, technical and agency reports, and patent preparations.
• Collaborates with senior staff to publish findings in refereed journals.
• Creates and conducts experiments and analyzes results.
• Works with other researchers to use and develop end products.
• Writes extramural proposals with approval from appropriate senior staff.
• Provides ready access to all experimental data for managing staff.
• Requests or acquires equipment and supplies for assigned project(s).
• Uses the Internet, surveys, legal documents, and other available sources to collect information.
• Applies for grants to continue research and prepares materials for submission to granting agencies and foundations.
• Adheres to University and unit-level policies and procedures and safeguards University assets.

This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary.

CORE QUALIFICATIONS

Education:

Ph.D., M.D. or other doctoral level degree required

Experience:

Minimum 1 year of relevant experience

Knowledge, Skills and Attitudes:
Skill in collecting, organizing, and analyzing data.
Ability to recognize, analyze, and solve a variety of problems.
Ability to exercise sound judgment in making critical decisions


Department Specific Functions


The Sylvester Comprehensive Cancer Center is looking for an Asst. Scientist to join the newly established Proteomic Shared Resources, to apply state of the art LC-MS based methods and techniques for quantitative proteomics.

As an Asst. Scientist, you will be responsible for the maintenance and operation of a modern high resolution orbitrap mass spectrometer as well as performing detailed biochemical sample processing techniques. In addition, you will be part of a small, agile, interdisciplinary team that seeks to invent innovative methods that can be used in the development of diagnostics aimed at getting the right treatment to right patients and understanding the fundamental underpinnings of cancer biology.

The individual will be:

• Primary responsible for the use of a new Orbitrap Ascend Tribrid LC-MS instrument for proteomic applications.
• Will manage inventory of lab consumables, including ordering, receiving, and storage of supply stocks.
• Prepare protein samples from a wide range of specimen (e.g., cells, tissues, biofluids, and SDS Page gels)
• Implement of both bottom-up & top-down proteomic approaches.

• Responsible for global proteome profiling by data-dependent (DDA) and data-independent (DIA) acquisition mass spectrometry and mass bar-codes. Detection of post-translational modifications (e.g., phosphorylation, acetylation).
• Perform relative quantification of proteins by label-free and stable-isotope labelling strategies (e.g., SILAC, TMT).
• Responsible for structural proteomics (e.g., cross-linking, pull-down experiments).
• Will conduct Single cell proteomics analysis.
• Intact protein analysis & MW determination.
• Use of proteomics software (e.g., Proteome Discoverer, MASCOT) for data analysis.
• Responsible for operation, maintenance, and troubleshooting of Orbitrap Ascend Tribrid Mass Spectrometry and other laboratory equipment as needed.
• Assesses experimental results and troubleshoot methods and instruments to optimize research protocols.
• Collaborates efficiently with technicians, graduate students, post-docs, PIs, the…