Research Associate , SOM

Position: Research Associate 3, SOM (A)
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The department of Medicine has an exciting opportunity for a full-time Research Associate 3 to work on the UHealth campus. The Research Associate 3 supports research activities under the direction of a principle investigator, program director or designee of the principle investigator or program director.

CORE JOB FUNCTIONS

* Maintains daily operations and performs procedures related to new and on-going research projects.

* Assists with ordering, purchasing, and maintenance of all required reagents and supplies for laboratory activities, and monitors and maintains inventory of supplies and stocks.

* Assists with the maintenance of institutional biosafety and animal care protocols, laboratory safety records, and standard operating procedures.

* Performs and assists other research staff with standard laboratory techniques.

* Develops, adapts, and implements new laboratory procedures.

* Records, organizes, and writes up experimental results for submission of manuscripts and for scientific presentations.

* Keeps abreast of latest literature in the field and searches for references to technical problems.

* Maintains current knowledge of the methods and techniques related to the specific field of research.

* Adheres to University and unit-level policies and procedures and safeguards University assets.

Department Specific Functions

* Independently design, perform, and troubleshoot experiments investigating mechanisms of pulmonary inflammation, immune regulation, tissue repair, and resolution biology.

* Perform murine models of lung injury and disease, including bacterial pneumonia, acute lung injury, ARDS, asthma, lung cancer, environmental exposure models, and sleep-related inflammatory models.

* Conduct animal procedures including intratracheal instillation, intranasal administration, bronchoalveolar lavage, tissue harvesting, blood collection, and survival studies.

* Isolate and characterize immune cell populations from lung, lymphoid tissues, blood, and bronchoalveolar lavage specimens.

* Design, optimize, and execute high-parameter spectral flow cytometry panels (25-40 colors) for comprehensive immune phenotyping.

* Perform cell sorting and downstream molecular analyses of macrophages, T regulatory cells, monocytes, neutrophils, dendritic cells, and other immune populations.

* Execute molecular biology techniques including RNA isolation, qPCR, bulk RNA sequencing sample preparation, single-cell RNA sequencing workflows, TCR sequencing, and gene expression analyses.

* Conduct cell culture experiments involving primary macrophages, T lymphocytes, epithelial cells, endothelial cells, and immortalized cell lines.

* Perform advanced immunologic assays including phagocytosis, cytokine profiling, proliferation assays, intracellular signaling studies, and functional immune cell characterization.

* Assist with spatial transcriptomics, confocal microscopy, immunofluorescence imaging, and image analysis.

* Maintain breeding colonies and perform genotyping of transgenic and conditional knockout mouse models.

* Analyze experimental data using Flow Jo, Prism, R, and other bioinformatic platforms.

* Maintain detailed laboratory records, experimental databases, standard operating procedures, and quality-control documentation.

* Prepare figures, datasets, protocols, abstracts, manuscripts, and grant-related materials for publication and presentation.

* Train and supervise students, technicians, fellows, and research staff in laboratory techniques and animal procedures.

* Coordinate laboratory operations including inventory management, equipment maintenance, biosafety compliance, IACUC protocols, and reagent procurement.

* Collaborate with investigators, core facilities, and external research partners to support multidisciplinary translational research projects.

This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or…