Research Associate , SOM

The department of Medicine has an exciting opportunity for a full-time Research Associate 3 to work on the UHealth campus. The Research Associate 3 supports research activities under the direction of a principle investigator, program director or designee of the principle investigator or program director.

• Maintains daily operations and performs procedures related to new and on-going research projects.

• Assists with ordering, purchasing, and maintenance of all required reagents and supplies for laboratory activities, and monitors and maintains inventory of supplies and stocks.

• Assists with the maintenance of institutional biosafety and animal care protocols, laboratory safety records, and standard operating procedures.

• Performs and assists other research staff with standard laboratory techniques.

• Develops, adapts, and implements new laboratory procedures.

• Records, organizes, and writes up experimental results for submission of manuscripts and for scientific presentations.

• Keeps abreast of latest literature in the field and searches for references to technical problems.

• Maintains current knowledge of the methods and techniques related to the specific field of research.

• Adheres to University and unit-level policies and procedures and safeguards University assets.

• Independently design, perform, and troubleshoot experiments investigating mechanisms of pulmonary inflammation, immune regulation, tissue repair, and resolution biology.

• Perform murine models of lung injury and disease, including bacterial pneumonia, acute lung injury, ARDS, asthma, lung cancer, environmental exposure models, and sleep-related inflammatory models.

• Conduct animal procedures including intratracheal instillation, intranasal administration, bronchoalveolar lavage, tissue harvesting, blood collection, and survival studies.

• Isolate and characterize immune cell populations from lung, lymphoid tissues, blood, and bronchoalveolar lavage specimens.

• Design, optimize, and execute high-parameter spectral flow cytometry panels (25-40 colors) for comprehensive immune phenotyping.

• Perform cell sorting and downstream molecular analyses of macrophages, T regulatory cells, monocytes, neutrophils, dendritic cells, and other immune populations.

• Execute molecular biology techniques including RNA isolation, qPCR, bulk RNA sequencing sample preparation, single-cell RNA sequencing workflows, TCR sequencing, and gene expression analyses.

• Conduct cell culture experiments involving primary macrophages, T lymphocytes, epithelial cells, endothelial cells, and immortalized cell lines.

• Perform advanced immunologic assays including phagocytosis, cytokine profiling, proliferation assays, intracellular signaling studies, and functional immune cell characterization.

• Assist with spatial transcriptomics, confocal microscopy, immunofluorescence imaging, and image analysis.

• Maintain breeding colonies and perform genotyping of transgenic and conditional knockout mouse models.

• Analyze experimental data using Flow Jo, Prism, R, and other bioinformatic platforms.

• Maintain detailed laboratory records, experimental databases, standard operating procedures, and quality-control documentation.

• Prepare figures, datasets, protocols, abstracts, manuscripts, and grant-related materials for publication and presentation.

• Train and supervise students, technicians, fellows, and research staff in laboratory techniques and animal procedures.

• Coordinate laboratory operations including inventory management, equipment maintenance, biosafety compliance, IACUC protocols, and reagent procurement.

• Collaborate with investigators, core facilities, and external research partners to support multidisciplinary translational research projects.

This list of duties and responsibilities is not intended to be all-inclusive and may be expanded to include other duties or responsibilities as necessary.

• Bachelor's degree in relevant field required.

• Minimum 5 years of relevant experience required.

• Master's degree in Immunology, Cell Biology, Molecular Biology, Biomedical Sciences, or related field preferred.

• AALAS certification preferred.

• Training and certification in animal handling and biosafety procedures are preferred.

• Flow cytometry and cell sorting experience strongly preferred.

• Learning Agility:
  Ability to learn new procedures, technologies, and protocols, and adapt to changing priorities and work demands.

• Teamwork:
  Ability to work collaboratively with others and contribute to a team environment.

• Technical Proficiency:
  Skilled in using office software, technology, and relevant computer applications.

• Communication:
  Strong and clear written and verbal communication skills for interacting with colleagues and stakeholders.

The University of Miami offers competitive salaries and a comprehensive benefits package including medical, dental, tuition remission and more.

UHealth-University of Miami Health System, South Florida's only university-based health…