Scientist I, PK​/PD Bioanalytical Assays, Immunology

Breeze Bio is a rapidly-growing, early-stage company developing a proprietary and novel delivery platform with the potential to transform genetic medicine. Our interdisciplinary team works together across chemistry, payload design, formulation, and screening and preclinical groups to advance our Nano Galaxy® hydrophilic nanoparticle (HNP) platform toward a pipeline of therapeutic candidates. Breeze Bio fosters an exciting and dynamic environment where we value impactful data and creative solutions.

We are seeking talented and motivated individuals to join our team as we expand across all functions within the organization. Join us in Brisbane, CA, to be at the forefront of the future of genetic medicine.

We are seeking a talented and motivated Scientist to join our Immunology team supporting non-clinical pharmacokinetic (PK), biodistribution, and pharmacodynamic (PD) evaluation of Breeze Bio's Nano Galaxy® hydrophilic nanoparticle (HNP) platform for nucleic acid–based therapeutics. This individual will report to the Principal Scientist leading PK/PD Bioanalytical Assays within the Immunology team and will be an individual contributor responsible for developing, executing, and interpreting quantitative bioanalytical and immune-monitoring assays that support IND-enabling non-clinical studies.

The ideal candidate will have hands-on experience with quantitative bioanalytical platforms in complex biological matrices, nucleic acid–based PK/biodistribution assays, and pharmacodynamic immune assays, gained in the context of emerging therapeutic modalities such as gene therapy and nucleic acid therapeutics. Individuals with a successful track record of independent bench execution, rigorous data analysis, and teamwork in a fast-paced biotech environment are preferred.

This is primarily a bench-based role (approximately 75% hands-on assay execution), spanning bioanalytical, pharmacodynamic, and biodistribution downstream assays, including:

• Bioanalytical assay execution: Develop, optimize, and independently execute quantitative bioanalytical assays — RT-qPCR, ddPCR, ELISA, and MSD — to quantify nucleic acid payload and protein analytes in complex biological matrices (serum, plasma, tissue homogenates) supporting PK, biodistribution, and toxicokinetic evaluations of mRNA-HNP and other nucleic acid–based therapeutics.
• Pharmacodynamic and immune-monitoring assays: Execute PD assays integrated with PK sample collection, including multicolor flow cytometry–based immune cell phenotyping, functional assays (e.g., IFNγ ELISpot/Fluoro Spot, Treg suppression assays), and cytokine multiplex profiling (MSD, CBA) to characterize antigen-specific immune activation, tolerance, and target engagement across in vivo studies.
• Study design and execution: Contribute to design, protocol development, and execution of in vivo downstream assays for PK, biodistribution, PD, and toxicology studies (Bioanalytical and PD assays) in rodent and other preclinical models supporting IND-enabling programs.
• Method development and rigor: Support bioanalytical method development, qualification, and troubleshooting in alignment with regulatory bioanalytical expectations; maintain assay robustness, reproducibility, and appropriate documentation for regulatory submissions.
• Data analysis and reporting: Independently analyze, interpret, and summarize PK, biodistribution, and PD data (e.g., non-compartmental PK parameters, tissue distribution profiles, immune modulation end-points); present results at team, cross-functional, and external meetings.
• Collaboration and communication: Significant interaction with Discovery Biology team in developing PD and Immune monitoring assays. Work cooperatively with peers across Invivo, and Formulation, in a highly matrixed lab environment; keep detailed and organized lab records/ELN entries.

• Ph.D. or equivalent in Pharmacology, Toxicology, Immunology, Molecular Biology, or a related life science field, with 0–2 years of relevant biotech/pharma industry or postdoctoral experience.
• Hands-on experience with quantitative bioanalytical assay platforms — RT-qPCR, ddPCR, ELISA, and…