Research Scientist, Proteomics

Job Title

Responsibilities and Duties

Corporate

Follow SOPs and other applicable laboratory or company procedures

Maintain confidential information consistent with Inotiv corporate policy

Demonstrate Inotiv Core Values

General

Interact with clients, other employees, and the community in a professional manner

Support and participate in company initiatives

Perform other duties as assigned

Specific

Following the safety rules in the lab and maintaining a safe working environment

Operating lab equipment and performing analyses according to the Operation Manuals, SOPs, Best Practices, and training

Performing proteomic sample preparation in support of discovery and translational studies, including:

Protein extraction and lysis, reduction and alkylation, and enzymatic (e.g., tryptic) digestion

Peptide cleanup and desalting (S-Trap, SP3, SPE) and protein/peptide quantification

Preparing buffers, reagents, and stock solutions, and verifying their composition

Preparing samples through LC-MS injection and assisting with data acquisition as instructed

Operating, programming, and maintaining automated liquid-handling and bead-based sample-preparation systems (e.g., King Fisher, Bravo) to support reproducible, plate-based, high-throughput proteomic processing

Recording results accurately in the ELN and contributing to data summaries and study documentation

Notifying the Lab Manager and/or Principal Investigator of any deviations from the study protocol/SOPs/Best Practices or unexpected processes preventing the proper completion of sample processing and documenting as required

Observing deadlines, producing timely results or communicating to management when deadlines cannot be accomplished

Performing assigned general lab tasks, including data logging, routine maintenance of equipment, and general housekeeping

Ordering reagents, parts and/or materials needed for the functioning of the Lab. Handling, inventorying and managing incoming materials and samples, waste and shipping of samples to outside parties when needed

Ensuring training files are properly maintained by keeping his/her CV current and for providing copies of certificates or documentation of training

Demonstrating an understanding of Best Practices and SOPs, and applying Good Laboratory Practice principles (21 CFR Part 58) where studies are conducted under regulatory requirements

Position Requirements

Education and experience

BS, or MS, or equivalent degree in Chemistry, Biochemistry, Biology, Molecular Biology, or related field with qualifying laboratory experience in degree program

Skills and abilities

A basic understanding of processes related to Bioanalysis including sample preparation, instrument / equipment operation and data processing and reporting

Experience with analysis of proteins and peptides by mass spectrometry, including protein-specific extraction, separation, and purification methods (e.g., in-solution or on-bead digestion, S-Trap, SP3, and C18/SPE peptide desalting)

Experience with, or aptitude to learn, automated sample-preparation platforms, such as the Thermo King Fisher (magnetic bead–based protein/peptide cleanup and SP3) and the Agilent Bravo / AssayMAP (automated liquid handling and cartridge-based peptide desalting)

Comfort working with plate-based, high-throughput workflows (e.g., 96-well processing) and large sample cohorts

An attitude for quality, an eye for detail, the ability to follow written instructions and work effectively with management to complete assigned tasks

Documentation discipline and the ability to follow SOPs and written protocols precisely; familiarity with GLP/GMP/GCP principles is a plus

Ability to use a computer and an electronic lab notebook (ELN) to record experimental detail, and to compile and maintain databases for records and inventory, utilizing the appropriate software

A working knowledge and awareness of general laboratory procedures

Good written and oral communication skills

Ability to adhere to all safety regulations and procedures

Desired:

Experience with software for analysis of mass spectrometry data

Performance Requirements

General

Ability to read, write, speak and understand English

Physical Activity

Moderate physical activity This position will require frequent walking, sitting and/or standing for extended periods of time, carrying, heavy lifting (max. 50 lbs), reaching while standing, gripping and twisting hand movements, finger control and other general repetitive motions (for more than four [4] hours per day)

Able to wear latex or nitrile gloves, dust mask or respirator, and safety glasses as required

Ability to read and understand applicable materials

Manual dexterity to operate laboratory equipment and calculator/computer

Able to read and interpret test results and instrument specifications

Working Conditions

Work is normally performed in a typical interior office work or laboratory environment

Work environment involves exposure to potentially dangerous materials and situations that require following extensive safety precautions and may…