Research Assistant II

Organization

PMI Molecular Pathogenesis

A laboratory in the Department of Pathology, Microbiology and Immunology is seeking a Research Assistant II (RAII) to join our team. In this role, you'll be a key laboratory resource—supporting day-to-day operations while contributing directly to ongoing research projects in a fast-paced, collaborative environment. With moderate supervision, you will set up and conduct experiments, follow established protocols, and ensure accurate documentation and reporting of results (including any unexpected or variant findings).

You will be part of the Vanderbilt Center for Antibody Therapeutics (VCAT), a world-leading center focused on antibody discovery and translational research. The team leverages computational and structure-based protein design to address fundamental questions in immunology and virology and to develop new vaccine and antibody product candidates targeting viruses of biomedical importance.

• Culturing cells (eukaryotic and prokaryotic)
• Performing molecular biology techniques such as transformations, DNA preparation, PCR, and sequence analysis
• Expressing and purifying proteins
• Performing flow cytometry
• Preparing common reagents and solutions
• Supporting routine maintenance of laboratory equipment
• Managing inventory of reagents and supplies
• Assisting with other laboratory and research activities as needed

• Under moderate supervision, conduct and evaluate experiments and assays
• Understand and follow experimental protocols (may include animal handling, tissue culture, etc.)
• Record, compile, interpret, and communicate data/results
• Follow University safety protocols (may include use of combustibles, radioactivity, toxic material, and/or carcinogens)
• Perform calculations involving molarity, % solutions, and serial dilutions
• Prepare, operate, and troubleshoot equipment in experimental procedures
• Read and follow all equipment manuals
• Maintain and repair equipment according to manufacturer and University guidelines
• Ensure laboratory is prepared for experiments
• Maintain inventories
• Maintain sterile conditions as required
• Prepare reagents
• Demonstrate an understanding of acceptable procedures and techniques
• Recognize problems and suggest solutions
• Assist in modifications as directed
• Provide appropriate written documentation of experiments
• Prepare preliminary research progress reports
• Summarize test results appropriately

Typical schedule is Monday-Friday, daytime hours, with flexibility in your daily start/end time as long as responsibilities are met. Occasional weekend work may be required but the anticipated work week is 40 hours. This is an on-site role working at VUMC Medical Research Building IV (MRB IV), 2215 Garland Ave., Nashville, TN.

• Bachelor's degree (or equivalent experience)
• 2 years of experience in molecular biology

Experience with plasmid preparation, cloning, DNA mutagenesis, and cultured mammalian cells.

This role offers the opportunity to make a meaningful impact within Vanderbilt Health, supported by a comprehensive benefits package which may include health, disability, retirement and/or wellness offerings to enhance your well-being and professional growth.

Vanderbilt Health is committed to fostering an environment where everyone has the chance to thrive and is committed to the principles of equal opportunity. EOE/Vets/Disabled.