Scientist II ~ Senior Scientist II, In Vivo

Nona Biosciences is a global biotechnology company committed to providing a total solution for partners worldwide, from academic institutions and biotech startups to biopharma giants. The integrated antibody discovery services range from antigen preparation, animal immunization, antibody discovery and screening, to lead generation and engineering, develop ability assessment and pharmacological evaluation, leveraging the advantages of Harbour Mice® platforms and the experienced therapeutic antibody discovery team.

Harbour Mice® generate fully human monoclonal antibodies in a two heavy and two light chain (H2L2) format, as well as a heavy chain only (HCAb) format. Integrating Harbour Mice® with a variety of antibody discovery platforms including single B cell cloning, hybridoma, surface display and direct CAR-based functional screening, Nona Biosciences is focused on driving global inventions of transformative next-generation biologics by diversified partnership strategies.

The values of the antibody discovery platforms and flexible partnership models have been well validated.

Nona Biosciences is seeking a skilled and experienced scientist to join our team in Natick, MA. The successful candidate will play a fundamental role in providing antibody discovery capabilities in our US lab, support internal and external therapeutics development programs. The ideal candidate will have extensive hands-on experience with single B cell screening and molecular biology techniques.

• Help to plan and execute mouse in vivo studies including immunizations, titer check and efficacy testing
• Lead antibody discovery efforts utilizing single B-cell screening technology.
• Execute mammalian cell transfections, lentiviral vector production, and cell transductions.
• Design and perform flow cytometry assays for target binding, immunophenotyping, and functional evaluation of immune cells.
• Conduct ELISA and MSD analyses to assess antibody responses in immunized animals and quantify cytokine production in primary cell-based assays.
• Perform in vitro assays, including primary human immune cell isolation, culture and differentiation
• Conduct independent data analysis contributing to scientific discussion and presentations
• Collaborate with other team members to support internal and external projects

• Master or PhD's degree in biology or a similar discipline
• 3+ years of hands-on experience with mammalian cell culture and molecular biology
• 3+ years of hands-on experience working with mice, especially euthanasia & tissue collection
• Some experience or knowledge of flow cytometry and ELISA is highly desired
• Excellent attention to detail, strong documentation skills, and ability to communicate effectively
• Excellent organization/planning skills to meet deadlines and handle multiple tasks simultaneously
• Quick ability to learn scientific knowledge and experimental techniques
• Diligent, self-motivated, independent and strong sense of responsibility
• Good interpersonal skills for building relationships across functions internally and externally