Lab Technician - Laboratory

Job Description

Lab Technician I - Laboratory

2439 Kuser Rd, Hamilton Township, NJ 08690, USA

Job Description

Laboratory Technician

Job Summary: Medical Diagnostic Laboratories(MDL), a member of Genesis Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses.

MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

MDL is looking for a Laboratory Technician. The Laboratory Technicians responsible for printing labels and attaching requisitions to the corresponding specimens.

Essential Functions:

Accessioning:

• Print labels and attach to requisitions and the corresponding specimens
• Properly accession multiple specimen types
• Take photographs of all specimens received
• Properly identify any discrepant information
• File all paperwork in order and distribute to the appropriate QC bins
• Properly scan accessioned requisitions
• Scan 2-D barcode elution tubes used for extraction
• Properly locate and record temperatures for all necessary stations

Manual Extraction:

• Perform Phenol/Chloroform manual extractions on biopsies and ticks
• Properly separate samples into their organic and inorganic layers via phenol/chloroform
• Properly perform quantitative analysis and hydrate all ticks and biopsies

Robotic Extraction:

• Properly extract all samples types using a provided XG sheet as a template
• Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of all necessary reagents
• Properly dispose of all waste material when required; both hazardous and non-hazardous
• Attend to robotics to address any user interactions and/or robotic malfunctions
• Manually extract all necessary samples via commercial kits

RNA Extraction:

• Perform manual RNA extractions via Qiagen kit for all necessary samples
• Perform robotic RNA extractions via VX Reagents kit for all necessary samples

PCR:

• Perform manual and robotic Conventional method PCR amplification
• Properly load and start thermocycler machines
• Perform manual and robotic Real-time method PCR amplification
• Properly load and start Rotor-gene thermocycler
• Perform initial analysis on completed Real-time PCR
• Perform Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor
• Properly load and start Bio-Rad CFX thermocycler
• Perform initial analysis on completed Acoustic Real-Time PCR

Gel Electrophoresis:

• Properly identify which completed PCRs from the thermocycler require gel electrophoresis
• Properly identify when to make and/or use 2% versus 2.5% gels
• Complete blank gel template sheets to combine short PCR sheets
• Use dye to stain all necessary samples with the appropriate volume on a test by test basis
• Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time)
• Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader
• Be able to troubleshoot any gel box or power source errors, as well as any gel reader errors

Pyrosequencing

• Properly perform nested PCR prior to sequencing
• Properly load and run Sequencing Analysis reactions (SQA)
• Properly load and run Single Nucleotide Polymorphism reactions (SNP)
• Properly analyze all run data

Micro-Biology/Cell Culture

• Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel;
  Streak for single…