Research Technician

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Job Summary We are seeking a highly motivated and detail-oriented Research Technician with extensive hands-on experience in insect cell culture (Sf9 and Hi5) using the baculovirus expression system and mammalian cell culture across diverse cell lines. The successful candidate will support ongoing structural and functional studies of protein complexes by producing high-quality recombinant proteins in both insect and mammalian systems. Essential

Job Duties Generate bacterial, insect cell, and mammalian expression constructs (basic molecular cloning techniques required). Maintain and passage insect cells (Sf9 and Hi5) and mammalian cell cultures under sterile conditions. Generate and amplify recombinant baculoviruses for protein expression in Sf9 and Hi5 cells. Transfect and maintain various mammalian cell lines (e.g., HEK
293, HTC
116, Vero, HeLa) for transient and stable expression. Optimize expression protocols for high-yield protein production in both systems. Perform small- and large-scale protein expression experiments. Harvest cells and assist with downstream, protein purification (modern chromatography methods). Perform immunofluorescence microscopy to assess protein localization. Conduct SDS-PAGE gel analysis and western blotting, as part of routine experiments. Maintain detailed records of all experiments, protocols, and cell line inventories.

Participate in general lab maintenance (organization, restocking, and generating shared reagents). Support researchers with other tasks as needed. Basic Qualifications Bachelor's degree in Biology, Biochemistry, Molecular Biology, or a related field. 2 years of hands-on experience with Sf9 and/or Hi5 cell culture and baculovirus-based protein expression. Proficiency in aseptic technique and routine mammalian cell culture. Familiarity with restriction enzyme cloning, western blotting, SDS-PAGE gel analysis, immunofluorescence microscopy, protein purification.

Strong organizational and communication skills. Ability to work independently and collaboratively in a team environment.

Preferred Qualifications Experience scaling up insect and/or mammalian cultures using flasks. Familiarity with serum-free media, virus titering, and MOI optimization. Understanding of basic molecular cloning and transfection techniques. Protein purification abilities are a plus but not necessary. Prior experience in academic or industrial protein production environments. Required Documents Resume