Generation Sequencing; NGS Senior Scientist

Job Description

We are seeking a highly motivated senior scientist to implement and lead our Next‑Generation Sequencing (NGS) platform as part of cell line development and genetic characterization for therapeutic proteins. The candidate will be a member of the Cell Engineering and Analytical Sciences group and play a key role in next‑generation technology development for biologics production cell lines.

• Operate, automate, qualify, and integrate the NGS platform into workflow, including staff training and documentation.
• Perform complex, high‑throughput NGS library preparation techniques (Illumina and Oxford Nanopore Technologies long read) and process DNA and RNA isolation, quantification, fragmentation, quality assessment, PCR, and ddPCR.
• Design, troubleshoot, and optimize complex NGS‑based algorithms.
• Integrate and analyze NGS data (differential expression, transcript characterization, integration mapping, and mutation detection).
• Use and visualize high‑content molecular data analysis tools.
• Develop tools for bench scientists and senior leaders to visualize departmental data.
• Identify trends in departmental data to drive process changes.
• Coordinate data aggregation from cross‑functional teams for identification of lead manufacturing cell lines.
• Design and direct research projects related to upstream process development, including metabolic/process modeling.
• Design experiments, analyze data, troubleshoot, provide detailed training and mentorship, contribute to project teams, and maintain detailed records for laboratory notebooks and technical documents.
• Present scientific data at internal and external meetings/conferences and publish results in peer‑reviewed journals.

• Minimum of aB.S. degree in Computer Science, Bioinformatics, Data Science, Chemical Engineering, Cell Biology, Molecular Biology, or related field with 12+years of progressively advanced data analysis experience; or aMaster’s with 6+years; or aPh.D. with 3+years.
• Strong bench skills with routine molecular biology lab techniques (nucleic acid extraction, PCR, ddPCR).
• Extensive knowledge of NGS platforms and library preparation (Illumina, Oxford Nanopore Technologies).
• Basic understanding or willingness to develop Unix/Linux command‑line scripting and high‑performance computing data‑management skills.
• Broad experience in genomics data analysis including differential expression, transcript characterization, and mutation detection.
• Ability to work in a fast‑paced environment and collaborate across functional groups.
• Excellent organizational, verbal, and written communication skills.

• Tecan Automation
• High‑content biological data management, analysis, and presentation
• Understanding of mammalian cell culture and protein expression and analysis
• Familiarity with bioreactor process and mammalian cell metabolism
• Analytical reasoning, biochemistry, chemistry, manufacturing and control (CMC), coaching, consulting, critical thinking, drug discovery development, emerging technologies, molecular diagnostics, pharmacogenetics, pharmacovigilance, process improvements, quality assurance (QA), relationship building, research documents, scientific research, technologically savvy

Johnson & Johnson is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, disability, protected veteran status, or other characteristics protected by federal, state, or local law. We actively seek qualified candidates who are protected veterans and individuals with disabilities as defined under VEVRAA and Section 503 of the Rehabilitation Act.

Johnson & Johnson is committed to providing an inclusive interview process for applicants’ needs. If you have a disability and would like accommodation, please contact us via the designated accommodation resource.