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Lentiviral Vector Upstream Process Development Scientist

Job in Ambler, Montgomery County, Pennsylvania, 19002, USA
Listing for: Insight Global
Full Time position
Listed on 2026-09-02
Job specializations:
  • Research/Development
    Research Scientist, Biotechnology, Biotech Research, Pharmaceutical Science/ Research
Job Description & How to Apply Below
Location: Ambler

Upstream Associate Scientist

Required

Skills & Experience:

Bachelor's or Master's degree in a relevant scientific discipline with 3–5 years of lab-based experience in upstream cell culture or bioprocessing.

  • Experience with lab techniques, including but not limited to aseptic cell culture and routine analysis of pH, gases, metabolites, cell densities, and viabilities.
  • Experience with large-scale bioreactors (wave or stirred-tank, single-use preferred)
  • Strong analytical skills with the ability to identify gaps, troubleshoot issues, and propose innovative solutions.
  • Microsoft Office proficiency, particularly with data aggregation and analysis.
  • Familiarity with LVV production, CAR T process development, and technical transfer activities.
  • Experience supporting scale-up or platform development initiatives in a regulated environment.
  • Experience with modeling software tools

Job Description:

Our large pharmaceutical client is seeking a motivated and detail-oriented Upstream Associate Scientist to support lentiviral vector (LVV) production within our small-scale upstream development team. This role focuses on platform process optimization, characterization, and technical transfer activities to advance our next-generation LVV modality supporting CAR T manufacturing for solid tumor indications.

Key Responsibilities:

-Execute routine suspension cell culture and aseptic preculture operations, including cell vial thawing, shake flask expansion, wave bag preparation, and bioreactor setup.

-Perform LVV production runs at scales ranging from 10 L to 50 L using single-use bioreactors, with a focus on sterility and process consistency.

-Conduct Am  and shake flask scale-down studies to support platform development and material generation.

-Management of experimental samples, including aliquoting, storage, and shipment as needed.

-Collect and analyze post-run data, generate technical reports, and present findings to cross-functional teams.

-Maintain accurate records of analytical data and ensure compliance with safety, quality, and regulatory standards.

-Collaborate with internal and external stakeholders to support onboarding of new processes and resolution of technical challenges.

-Contribute to ongoing improvements in the existing CAR T process and support development of new LVV platforms aligned with FDA CAR T expectations.

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