Research Specialist D

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Posted Job Title

Research Specialist D

Job Profile Title

Research Specialist D

Job Description Summary

This Research Specialist D position will advance new therapeutics towards the clinic and contribute to the scientific work that will be published from the lab. Its focus will be testing new engineered T cells, evaluating their functional activity as a single agent or in combination to enhance therapeutic T cell performance, particularly in tumor microenvironments. This position will require strong immunological skills including CAR T cell manufacturing, flow cytometry, ELISA, and in vitro assays, as well as in vivo mouse studies, with a goal to enhance the therapeutic index of these therapies and understand their resulting impacts within the tumor microenvironment.

The primary function of this Research Specialist D position will be to perform in a supportive and independent manner in vitro and in vivo mouse studies, colony maintenance, and day-to-day management of the research bench, all carried out for the BRITE Lab under the direct supervision of Nour Shobaki. The purpose of this position is to establish mouse studies by generating and characterizing engineered immune therapeutics, growing and developing tumor cells, performing injections, monitoring animal health, weight, and therapeutic responses, collecting datasets from the study (tumor growth, therapeutic engraftment, histology, ex vivo assays), and contributing to the evaluation, presentation, and publication of results.

Job Description

Job Responsibilities

+ Perform immunological functional assays in vitro including CAR T cell manufacturing, flow cytometry, and ELISA. Evaluate immune therapies using established protocols and contribute to development of new methods implementing state-of-the-art techniques

+ Perform immunological functional assays in vivo. Execute mouse studies using established protocols and contribute to development of new protocols under the direction of the lab supervisor.

+ Modify and prepare tumor cells and engineered immune therapies for in vivo mouse experiments, documenting their characteristics and performing assays defining functional performance.

+ Monitor and collect datasets associated with animal studies including health monitoring and weights, tumor growth (caliper, bioimaging), bleeds, and necropsies. Perform IV, IT, IP, and SC injections of tumor cells and therapeutics.

+ Prepare tissues at necropsy by fixing and isolating cells for functional and flow cytometric analysis, IHC, and spatial transcriptomic studies.

+ Document experimental data, perform data analysis, and prepare presentations of results under the guidance of the lab director for lab meetings and manuscript contributions.

+ Manage day-to-day research bench operations…