Research Technician II- Diamond Lab

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This entry-level research technical role participates in a planned research projects by performing research protocols, experimental procedures and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in execution and interpretation of experiments, and of data derived.

We are a hepatology lab in the Abramson Building at CHOP focused on dysregulated immune responses that result in liver failure in children. As part of understanding these diseases in the liver we collect patient samples during procedures and work with mouse models to find treatments for these disorders. Dr. Diamond is an attending hepatologist and investigator with a research interest in how inflammatory molecules called cytokines cause liver injury and dysfunction.

His lab works closely with a larger group at CHOP focused on immune dysregulation disorders in various disciplines including neonatology, rheumatology and gastroenterology.

We work primarily with mouse models of human diseases such as Hemophagocytic Lymphohistiocytosis, Macrophage Activation Syndrome and acute immune mediated hepatitis. We also perform experiments on patient samples to correlate what we study in mice. Some of the techniques we use include flow cytometry, cell sorting, qPCR, microscopy, single cell next generation sequencing, spatial transcriptomics, and ELISA assays amongst others. This position will receive extensive training in these techniques during your time in our lab.

You will have an important role in the lab's mission to further the understanding of the interaction between the immune system and liver function. We are a jovial bunch that collaborates with multiple other labs at CHOP and we are looking for a motivated future member to join our scientific discovery and fun lunches. Our lab currently includes two lab technicians who will help train you when you join the group, as well as a bioinformatics specialist.

In our larger group there are multiple members in various levels of scientific training who are always happy to help and work together on projects. We aspire to be a lab full of kind, collaborative, and motivated individuals with diverse backgrounds and experiences.

• Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
• Performs research procedures on experimental samples as directed
• Records and checks experiments/ results for completeness and accuracy
• Performs calculations and maintains appropriate log and lab notebooks Performs tasks that require familiarity with fundamental techniques and lab equipment
• May assist with planning specific research protocols and procedures
• Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures) Assists in care and maintenance of lab equipment
• May coordinate schedules of tests and procedures
• Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
• Records results, organizes data and maintains lab notebook in an organized manner
• Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
• Conducts literary searches as directed