Senior Research Scientist

Senior Research Scientist

The University of Pittsburgh's School of Medicine, Department of Internal Medicine, Division of Rheumatology and Clinical Immunology seeks a Senior Research Scientist in Pittsburgh, PA.

Duties include:

independently leading scientific research projects and supervising trainees and research activities; high-level molecular biology, including sequencing and sample/library preparation; working with murine models of immune dysregulation, including asthma, neuroinflammation, autoinflammation, infection, and cardiovascular disease; immune cells functional assays; cloning and transfection; and screening primary patient cells for disease-causing mutations. Must have a PhD degree (or foreign equivalent degree) in Biology, Immunology or a related field plus three years of experience in a related position.

Experience must include three years working with: human immunology research, including primary human immune cell isolation, culture, stimulation, gene editing, and functional analysis; murine models of inflammation, allergy, and atherogenesis, including designing and conducting in vivo experiments and processing a wide range of tissues for immunologic analysis, histological analysis, and specialized staining; multiparameter flow cytometry, including experimental design, panel development, compensation, gating strategies, and data analysis;

molecular biology techniques, including nucleic acid extraction, qPCR, ddPCR, RNA-seq; gene-expression manipulation using lentiviral systems, including viral particles production and transduction for stable knockdown, knockout, or over-expression; functional in vitro assays with mammalian cell lines, including evaluation of cell activation, viability, proliferation, cytokine production, etc; and handling, shaving, and injecting (IV, IP, intradermal) mice and processing broad range of tissues.

Experience can be concurrent.