Scientist II

Job Description
Our client, a world leader in diagnostics and life sciences, is looking for a "
Scientist II
" based out of Pleasanton, CA.

Job Duration:
Long term Contract (Possibility Of Further Extension)

Pay Rate: $55/hr on W2

Company Benefits:
Medical, Dental, Vision, Paid Sick leave, 401K

The Bio Specimen Acquisition and Management function is a centralized service function which supports the acquisition, characterization, and management of biological materials, as well as build of select panels for clinical research and cultivation and characterization of various Microorganisms and Mammalian cell lines. The Scientist II position will facilitate the daily operations associated with fulfilling service requests for Culture Collection and panel activities, while providing best-in-class simple biological solutions to our customers to drive innovation.

Responsibilities:

• Under minimal supervision, executes assigned project activities using established procedures and applying scientific knowledge and accumulated experience to complete projects involving manipulating human cell lines, microorganism and/or other biological material.
• Perform standard recombinant DNA techniques including cloning, restriction digestion, ligation, and transformation of bacteria.
• Grow bacterial cultures under sterile conditions to ensure successful transformation and plasmid propagation.
• Analyze plasmid DNA and RNA transcripts for purity, concentration, and quality.

Qualifications:

• Bachelor's degree in Molecular Biology, Cellular Biology, or Microbiology or related field and 4+ years of relevant experience.
• Master's Degree in Molecular Biology, Cellular Biology, or Microbiology or related field and 2+ years of relevant experience.
• Experience with aseptic techniques and bacterial culture or cell culture.
• Must be able to manually manipulate samples (plating, pipetting, labeling and vialing) on a routine basis.
• Experience with cloning techniques including plasmid design and preparation, restriction enzyme digestion, ligation and bacterial transformation and processing.
• Experience in performing nucleic acid extractions (DNA/RNA), quantification, amplification and characterization using Qubit, Nanodrop, Gel electrophoresis, PCR machines etc.
• Must be able to work in a laboratory environment on a daily basis under BSL-2 laboratory conditions. May work a variety of infectious human material (HxV, pathogenic bacteria, or engineered material, etc) and sample types (blood, serum, urine, stool etc).
• Experience with LIMS software, eLN and JMP (Preferred).

If interested, please send us your updated resume at