Senior Scientist, Protein Expression

Senior Scientist

My client is seeking a Senior Scientist to join their team and lend expertise in cell-free protein expression in bacterial systems.

My client offers the independence, resources, and collaborative environment needed to drive innovative research.

The ideal candidate will take on the following tasks:

• Design, execute, optimize, and troubleshoot bacterial cell-free protein expression for a diverse range of target proteins.
• Optimize extract preparation methods to maximize protein yield and activity.
• Develop and apply high-throughput screening approaches to evaluate expression constructs, chaperone combinations, and reaction conditions.
• Characterize expressed proteins for yield, purity, solubility, folding, and functional activity using SDS-PAGE, capillary electrophoresis, ELISA, and related analytical techniques.
• Maintain detailed lab notebooks and contribute to internal reports, SOPs, and patent disclosures.
• Stay current with advances in cell-free protein synthesis literature and evaluate new methods, vendors, and platforms.

The ideal candidate will possess the following qualities:

• PhD in Biochemistry, Molecular Biology, Protein Science, or related.
• Demonstrated hands-on expertise in bacterial cell-free protein synthesis including optimization and troubleshooting.
• Strong working knowledge of
  
  E. coli molecular biology, protein expression, and extract preparation.
• Proficiency in standard protein analytical techniques (SDS-PAGE, Western blot, ELISA, capillary electrophoresis).
• Experience with high-throughput and automated screening platforms.
• Proven ability to lead scientific projects and collaborate across multidisciplinary teams.

My client offers a base salary of $115k-135k plus bonus and relocation.