Scientist, Research Scientist, Biomedical Science

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH).

Axle is seeking a Scientist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Child Health and Human Development (NICHD) located in Bethesda, MD.

Benefits We

Offer:

• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus
• Flexible Spending Accounts:
  • Healthcare (FSA)
  • Parking Reimbursement Account (PRK)
  • Dependent Care Assistant Program (DCAP)
  • Transportation Reimbursement Account (TRN)

Position Overview

Axle is seeking a Scientist to support the planning and execution of research experiments within the laboratory's overall research program, performing protein assays, transfection experiments, DNA cloning, and phenotyping of mutant mouse strains, along with protein function assays and mouse colony maintenance. This role supports laboratory staff on research projects and techniques, including mentoring post-baccalaureate fellows and assisting post-doctoral fellows with experiments in protein biochemistry, and evaluates, analyzes, and interprets research data, communicating results and methods in reports, presentations, and manuscripts.

A Master's degree in Biology, Life Sciences, or a related discipline is required, along with extensive experience as a lead investigator on research projects.

Certifications & Licenses

• Master's degree in Biology, Life Sciences, or a related discipline

Field of Study

• Genetics

Software

• Power Point
• Outlook
• Excel
• Word

Skills

• Extensive experience as lead investigator on research projects
• Work products and documents related to performing protein assays, phenotyping of mutant mouse strains, protein functional assays, and other less common assays; performing transfection experiments with plasmids and retroviruses into cell lines or primary mouse cells; and DNA cloning and generation of constructs for gene expression and gene targeting in ES cells
  - Ad-Hoc
• Work products and documents related to preparing media, buffers, and chemical reagents; maintaining lab supplies and equipment; and assisting post-doctoral fellows in their own independent projects through consultation and by performing selected key experiments within the contractor's area of expertise
  - Ad-Hoc
• Work products and documents related to performing key pilot experiments to test new hypotheses forming the foundation of new research projects; performing cell culture and fetal thymus organ culture; and maintaining a mouse colony for experiments
  - Ad-Hoc
• Work products and documents related to analyzing and summarizing data for reports; assessing project direction, performing literature searches, and assisting with identification of new projects and research directions to pursue; and participating in meetings and discussions
  - Ad-Hoc
• Meet with lab members to present updates
  - Weekly

Statement of Work Details

• Perform protein assays by Western Blotting to detect protein expression and protein associations in T cells from mice - 1
• Perform transfection experiments with plasmids and retroviruses into cell lines or primary mouse cells - 2
• Perform DNA cloning and generate constructs for gene expression and gene targeting in ES cells - 3
• Support the planning and execution of research experiments in the context of the laboratory's overall research program
• Perform phenotyping of mutant mouse strains by multicolor flow cytometry
• Perform protein function (signaling) assays on thymocytes and T cells
• Perform and optimize less common assays, such as phosphatase assay and GTPase assay
• Perform cell culture and fetal thymus organ culture
• Maintain a mouse colony for…