Senior Research Technician - Molecular Microbiology

Location: St. Louis

## Senior Research Technician - Molecular Microbiology Apply remote type:
On Campus/Onsite locations:
Washington University Medical Campus time type:
Full time posted on:
Posted Todayjob requisition :
JR95222#
** Scheduled Hours
** 40#
** Position Summary
** The Kanjee lab studies how malaria parasites infect human red blood cells (RBCs). We are interested in identifying host receptors required by the parasite during RBC invasion, with a specific focus on the understudied parasite Plasmodium vivax. We use red blood cell genetics approaches (CRISPR/Cas9 knockout/knockdown & gene complementation) to understand the functional mechanisms of candidate host receptors during parasite invasion.
This position in the Kanjee lab will assist with experiments involving general molecular cloning, designing CRISPR/Cas9 knockout, knockdown and host receptor gene complementation constructs, in vitro culturing of primary and immortalized erythroid cells, culturing of malaria parasites, validation of host RBC genetic knockouts/knockdowns/complementation via flow cytometry and Western blot. These experiments will involve: collection of data, preparation of cultures, media and set-up and maintenance of equipment.
Performs independently complex research studies, experiments and assays.#
** Job Description
**** Primary

Duties & Responsibilities:

*** Performs complex analysis/projects according to research protocols, explaining methods and procedures to other technicians.
* Enters data and maintains current data files on research projects. Monitors data to ensure consistent quality.
* Prepares tissue cultures, reagents, probe tests, etc
* May perform preliminary statistical analysis of data.
* Assures compliance with established safety procedures and maintains required documentation on laboratory and specimen conditions.
* Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
* Assists in the training and development of technicians, acting as a group leader.
* Performs other duties as assigned.
*
* Working Conditions:

*
* * Works in a laboratory environment with potential exposure to biological and chemical hazards.
* Must be physically able to wear protective equipment and to provide standard care to research animals.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.#
** Required Qualifications
***
* Education:

** Master’s degree or combination of education and/or experience may substitute for minimum education.
** Certifications****/Professional Licenses****:
** No specific certification/professional license is required for this position.
*
* Work Experience:

** Laboratory Setting (1 Year)
*
* Skills:

** Not Applicable
** Driver's License:
** A driver's license is not required for this position.#
** More About This Job
***
* Preferred Qualifications:

*** Prior experience with
* in vitro
* cell culture in BSL2 work environment is an asset.
* Prior experience with mammalian genetics approaches and molecular cloning is an asset.
* Prior experience with parasite culture is an asset.
* Working knowledge of spreadsheets and statistical analyses.
* Excellent record keeping, organizational skills, and ability to interact with others.#
** Preferred Qualifications
***
* Education:

** No additional education unless stated elsewhere in the job posting.
** Certifications****/Professional Licenses****:
** No additional certification/professional licenses unless stated elsewhere in the job posting.
*
* Work Experience:

** No additional work experience unless stated elsewhere in the job posting.
*
* Skills:

** Communication, Laboratory Instrumentation, Recordkeeping, Software Program, Working Independently#
** Grade
* * R08-H#
** Salary Range**$18.77 - $31.94 / Hourly The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget.#
** Questions
* * For frequently asked questions about the application process, please refer to our External Applicant FAQ.#
** Accommodation
* * If you are unable to use our online application system and would like an accommodation, please email Candidate Questions or call the dedicated accommodation inquiry number at  and leave a voicemail with the nature of your request.

All qualified individuals must be able to perform the essential functions of the position satisfactorily and, if requested, reasonable accommodations will be made to enable employees with disabilities to perform the essential functions of their job, absent undue hardship.#
** Pre-Employment Screening
** All external…