Research Technician II - Molecular Microbiology

Location: St. Louis

## Research Technician II - Molecular Microbiology Apply remote type:
On Campus/Onsite locations:
Washington University Medical Campus time type:
Full time posted on:
Posted Todayjob requisition :
JR95278#
** Scheduled Hours
** 40#
** Position Summary
** The Kanjee lab studies how malaria parasites infect human red blood cells (RBCs). We are interested in identifying host receptors required by the parasite during RBC invasion, with a specific focus on the understudied parasite Plasmodium vivax. We use red blood cell genetics approaches (CRISPR/Cas9 knockout/knockdown & gene complementation) to understand the functional mechanisms of candidate host receptors during parasite invasion.
This position in the Kanjee lab will assist with experiments involving general molecular cloning, designing CRISPR/Cas9 knockout, knockdown and host receptor gene complementation constructs, in vitro culturing of primary and immortalized erythroid cells, culturing of malaria parasites, validation of host RBC genetic knockouts/knockdowns/complementation via flow cytometry and Western blot. These experiments will involve: collection of data, preparation of cultures, media and set-up and maintenance of equipment.
Assists with the technical aspects of studies and experiments, including documentation and preparation of materials.#
** Job Description
**** Primary

Duties & Responsibilities:

*** Assists with research studies, experiments and assays, including collection of data, preparation of solutions and setup and maintenance of equipment.
* Performs data entry and maintains data files on research.
* Prepares tissue cultures, reagents, probe tests, etc.
* Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
* Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
* Assists with general lab maintenance and cleaning.
* Performs other duties as assigned.  
*
* Working Conditions:

*
* * This position works in a laboratory environment with potential exposure to biological and chemical hazards.
* The individual must be physically able to wear protective equipment and to provide standard care to research animals.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.#
** Required Qualifications
***
* Education:

** Bachelor’s degree or combination of education and/or experience may substitute for minimum education.
** Certifications****/Professional Licenses****:
** No specific certification/professional license is required for this position.
*
* Work Experience:

** No specific work experience is required for this position.
*
* Skills:

** Not Applicable
** Driver's License:
** A driver's license is not required for this position.#
** More About This Job
***
* Preferred Qualifications:

*** The ideal candidate will have up to one year of experience in a laboratory setting.
* Prior experience with
* in vitro
* cell culture in BSL2 work environment is an asset.
* Prior experience with mammalian genetics approaches and molecular cloning is an asset.
* Prior experience with parasite culture is an asset.
* Working knowledge of spreadsheets and statistical analyses.
* Excellent record keeping, organizational skills, and ability to interact with others.#
** Preferred Qualifications
***
* Education:

** No additional education unless stated elsewhere in the job posting.
** Certifications****/Professional Licenses****:
** No additional certification/professional licenses unless stated elsewhere in the job posting.
*
* Work Experience:

** No additional work experience unless stated elsewhere in the job posting.
*
* Skills:

** Communication, Detailed Reports, Laboratory Instrumentation, Laboratory Research, Software Program, Working Independently#
** Grade
* * R06-H#
** Salary Range**$16.92 - $26.44 / Hourly The salary range reflects base salaries paid for positions in a given job grade across the…