UCSD Postdoctoral Scholar: Transforming Targeted NGS Drug-Resistant TB

Job Title: UCSD Postdoctoral Scholar:
Transforming Targeted NGS for Drug-Resistant TB

Job Number: 116402

Location: San Diego, US

The University of California, San Diego, Division of Pulmonary, Critical Care, Sleep Medicine, and Physiology in the Department of Medicine is recruiting a Postdoctoral Researcher to join the Rodwell/Colman Laboratory. The selected candidate will work on an NIH funded project to advance a novel targeted NGS assay aimed at making culture-free drug-resistance profiling faster, simpler, and more scalable on Illumina benchtop platforms.

As a Postdoctoral Scholar, working primarily on-site in La Jolla, San Diego, you will lead the process of designing and executing hypothesis-driven experiments that move the assay from promising prototype to rigorously characterized, automation-ready method. The role is embedded in an academic BSL-2 wet lab with close collaboration with clinical, engineering/automation and bioinformatics partners.

This position provides an opportunity to contribute to translational research with immediate relevance to global tuberculosis and antimicrobial resistance (AMR) control efforts at the interface of assay development, clinical application, and laboratory automation. You will be co-mentored by experienced investigators and thought leaders in diagnostic assay development and clinical studies, with opportunities to engage in World Health Organization-aligned initiatives.

In year one, the postdoctoral scholar will lead optimization of a multiplex amplicon panel through empirical primer-pool balancing and targeted redesign within approved frameworks, and transition defined assay steps from bench to laboratory automation integrated with established sample-preparation/normalization workflows. Work will include evaluation of manual and automated workflows on an Illumina iSeq
100 platform, partnership with bioinformatics colleagues on run-level metrics, and rigorous laboratory and digital documentation, with contributions to methods publications and internal and external academic presentations.

Applicants must have a PhD, MD/PhD, or equivalent degree in molecular biology, bioengineering, microbiology, or a related field at the time of application.

A successful applicant will demonstrate creativity, productivity, and strong interest in scientific method development through prior research experience.

• Hands-on expertise with primer design, and/or multiplex PCR and/or NGS library preparation.
• Demonstrated ability to plan, run, and interpret wet-lab molecular optimization experiments (e.g. fluorometric quantification, fragment analysis, benchtop sequencing operations).
• Strong documentation discipline.
• Independent but collaborative mindset across wet-lab and computational teams.
• Clear written and verbal communications.

The following experience is preferred but not required:

• Prior experience with Illumina sequencing workflows and instruments.
• Prior work balancing multiplex panels or migrating assays onto lab automation.
• Familiarity with primer design/assessment tools (Primer3, BLAST, Oligo Analyzer) and spreadsheet/scripting-level handling of metrics.
• Experience in BSL-2 workflows with human-derived materials; strict adherence to Environment, Health & Safety (EH&S) practices.
• Background in tuberculosis, antimicrobial resistance, or clinical genomics.

• Curriculum Vitae – Most recently updated C.V.
• Cover Letter including a 4-6 sentence description of a multiplex panel or primer design effort you led, the iterative parameter(s) you changed, and how you measured improvement.
• Statement of Research experience.

• 3 required (contact information only)

The University of California, San Diego is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, covered veteran status, or other…