Scientist -Antibody Technologies - Exp in Protein expression, Tissue culture, Molecular biology

Position :
Scientist
-Antibody Technologies
- Required Exp in Protein expression, Tissue culture, Molecular biology techniques

Location :
San Diego, CA

Duration : 12 Months

Total Hours/week : 40.00

pay rate:$20-30/hr

1st Shift

Client :
Medical Devices Company

Job Category :
Research & Development

Level of Experience :
Entry
- Level

Employment Type :
Contract on W2 (Need US Citizens or GC Holders or GC EAD or OPT or EAD or CPT)

Work hours:

40 hrs/wk. standard work hours ~8am-5pm.

3 Must haves on the resume: min 3 years lab experience. Protein expression experience. Tissue culture experience. Molecular biology techniques.

• We are seeking a highly motivated and organized Scientist III to contribute to the discovery and development of monoclonal antibodies in our Antibody Discovery team.
• This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to protein expression and purification, cell-line engineering and testing new antibodies for use in flow cytometry application.
• The position requires extensive hands-on experience with protein expression, tissue culture, and a strong understanding of biochemistry and molecular biology techniques.
• The successful candidate is expected to set up and execute experiments, be detail-oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results.

• Responsible for transfection and expression of antibodies and recombinant proteins using multiple cell lines and expression systems.
• Responsible for sterile cell culture of multiple cell lines using aseptic technique for expression and flow testing.
• Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.
• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and Flow Jo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

• Protein expression using various systems including but limit to mammalian cells, insect cells, and bacteria.
• Proficient with aseptic technique and cell-culture techniques.
• Protein purification with liquid chromatography and quality assurance using various analytical methods.
• Preferred:
  Familiarity with assay automation and automated liquid handlers.
• Preferred:
  Experience with cell line engineering.
• Preferred:
  Hand-on experience with Flow Cytometry and the ability of analyzing flow data.
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Effective analytical and problem-solving skills.

• A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 3-6 years of laboratory experience required.
• Protein expression, molecular biology and/or tissue culture experience required.