Gene Therapy Scientist : Cell Cloning & IVT

San Diego, United States | Posted on 05/21/2026

Our client, a world leader in life sciences and biotechnology, is looking for a“Scientist I” based out of
San Diego, CA.

Job Duration:

Long Term Contract(Possibility Of Extension)

Pay Rate : $46.43/hr onW2

Company Benefits:

Medical, Dental, Vision, Paid Sick leave, 401K

The Scientist I will bea vital contributor to our non-viral cell and gene therapy delivery platform,primarily focused on R&D for innovative nucleic acid payload technologies.

This role involves evaluating novel mRNA and DNA designs using a range ofmolecular biology techniques, including plasmid cloning, in vitro validation,and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR,and luciferase reporter assays.

Job Responsibilities:

· Design and clone expression vectors and IVT donor plasmids to support research initiatives.

· Execute DNA and mRNAtransfections in mouse and human cell lines.

· Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

· Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

• Bachelor'sdegree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate,site-directed mutagenesis) and IVT.
• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays andqRT-PCR.
• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g.,T cells, monocytes, hepatocytes).
• Desired proficiency in Snapgene, Prism, and MS Office.

If interested, pleases end us your updated resume at